View source for 6ppf


==Bacterial 45SRbgA ribosomal particle class B==
<SX load='6ppf' size='340' side='right' viewer='molstar' caption='[[6ppf]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ppf]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PPF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PPF FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ppf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ppf OCA], [http://pdbe.org/6ppf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ppf RCSB], [http://www.ebi.ac.uk/pdbsum/6ppf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ppf ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/A0A063XHU6_BACIU A0A063XHU6_BACIU]] This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.[HAMAP-Rule:MF_00402][RuleBase:RU000559] [[http://www.uniprot.org/uniprot/A0A063X754_BACIU A0A063X754_BACIU]] Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.[HAMAP-Rule:MF_01367][RuleBase:RU003950] [[http://www.uniprot.org/uniprot/A0A063XIS2_BACIU A0A063XIS2_BACIU]] This protein binds to 23S rRNA in the presence of protein L20.[HAMAP-Rule:MF_01363][RuleBase:RU000562][SAAS:SAAS00352917] [[http://www.uniprot.org/uniprot/F5HRS7_BACIU F5HRS7_BACIU]] One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.[HAMAP-Rule:MF_01326]  One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.[HAMAP-Rule:MF_01326] [[http://www.uniprot.org/uniprot/A0A063XCH0_BACIU A0A063XCH0_BACIU]] This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.[HAMAP-Rule:MF_01366][RuleBase:RU003878][SAAS:SAAS00725369] [[http://www.uniprot.org/uniprot/A0A063XCB6_BACIU A0A063XCB6_BACIU]] One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.[HAMAP-Rule:MF_01320] [[http://www.uniprot.org/uniprot/A0A063XFC1_BACIU A0A063XFC1_BACIU]] Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.[HAMAP-Rule:MF_00382][RuleBase:RU000560] [[http://www.uniprot.org/uniprot/F5HRT3_BACIU F5HRT3_BACIU]] The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.[HAMAP-Rule:MF_01331]  This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g., L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.[HAMAP-Rule:MF_01331][RuleBase:RU004008] [[http://www.uniprot.org/uniprot/A0A063XB36_BACIU A0A063XB36_BACIU]] One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.[HAMAP-Rule:MF_01369] [[http://www.uniprot.org/uniprot/A0A063XCP1_BACIU A0A063XCP1_BACIU]] Binds to the 23S rRNA.[HAMAP-Rule:MF_01341][SAAS:SAAS00124822] [[http://www.uniprot.org/uniprot/A0A063X745_BACIU A0A063X745_BACIU]] One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.[HAMAP-Rule:MF_01325][RuleBase:RU003906] [[http://www.uniprot.org/uniprot/F5HRS9_BACIU F5HRS9_BACIU]] This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.[HAMAP-Rule:MF_01337] [[http://www.uniprot.org/uniprot/A0A063X8U6_BACIU A0A063X8U6_BACIU]] Forms part of the polypeptide exit tunnel.[HAMAP-Rule:MF_01328]  One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.[HAMAP-Rule:MF_01328] 

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
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</SX>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Basu, K]]
[[Category: Britton, R A]]
[[Category: Guarne, A]]
[[Category: Jahagirdar, D]]
[[Category: Jain, N]]
[[Category: Ni, X]]
[[Category: Ortega, J]]
[[Category: Razi, A]]
[[Category: Seffouh, A]]
[[Category: 50s subunit]]
[[Category: Rbga]]
[[Category: Ribosome]]
[[Category: Ribosome assembly]]
[[Category: Ylqf]]