View source for 6agb


==Cryo-EM structure of yeast Ribonuclease P==
<SX load='6agb' size='340' side='right' viewer='molstar' caption='[[6agb]], [[Resolution|resolution]] 3.48&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6agb]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_(strain_atcc_204508_/_s288c) Saccharomyces cerevisiae (strain atcc 204508 / s288c)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AGB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6AGB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_P Ribonuclease P], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.5 3.1.26.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6agb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6agb OCA], [http://pdbe.org/6agb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6agb RCSB], [http://www.ebi.ac.uk/pdbsum/6agb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6agb ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/POP6_YEAST POP6_YEAST]] Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.<ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/POP3_YEAST POP3_YEAST]] Required for processing of 5.8S rRNA (short form) at site A3 and for 5'- and 3'-processing of pre-tRNA.<ref>PMID:9029160</ref> <ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/POP7_YEAST POP7_YEAST]] Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.<ref>PMID:9618478</ref> <ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/RPP1_YEAST RPP1_YEAST]] Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.<ref>PMID:9308968</ref> <ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/POP1_YEAST POP1_YEAST]] Required for processing of 5.8S rRNA (short form) at site A3 and for 5' and 3' processing of pre-tRNA.<ref>PMID:7926742</ref> <ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/POP8_YEAST POP8_YEAST]] Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.<ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/POP4_YEAST POP4_YEAST]] Required for 5.8S rRNA and tRNA processing; associated with RNase MRP and RNase P.<ref>PMID:9085845</ref> <ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/RPR2_YEAST RPR2_YEAST]] Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.<ref>PMID:9620854</ref>  [[http://www.uniprot.org/uniprot/POP5_YEAST POP5_YEAST]] Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.<ref>PMID:9620854</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-tRNA. Here we report the 3.5-A cryo-EM structures of Saccharomyces cerevisiae RNase P alone and in complex with pre-tRNA(Phe) The protein components form a hook-shaped architecture that wraps around the RNA and stabilizes RNase P into a "measuring device" with two fixed anchors that recognize the L-shaped pre-tRNA. A universally conserved uridine nucleobase and phosphate backbone in the catalytic center together with the scissile phosphate and the O3' leaving group of pre-tRNA jointly coordinate two catalytic magnesium ions. Binding of pre-tRNA induces a conformational change in the catalytic center that is required for catalysis. Moreover, simulation analysis suggests a two-metal-ion SN2 reaction pathway of pre-tRNA cleavage. These results not only reveal the architecture of yeast RNase P but also provide a molecular basis of how the 5'-leader of pre-tRNA is processed by eukaryotic RNase P.

Structural insight into precursor tRNA processing by yeast ribonuclease P.,Lan P, Tan M, Zhang Y, Niu S, Chen J, Shi S, Qiu S, Wang X, Peng X, Cai G, Cheng H, Wu J, Li G, Lei M Science. 2018 Sep 27. pii: science.aat6678. doi: 10.1126/science.aat6678. PMID:30262633<ref>PMID:30262633</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6agb" style="background-color:#fffaf0;"></div>

==See Also==
*[[RNase P|RNase P]]
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Baker's yeast]]
[[Category: Large Structures]]
[[Category: Ribonuclease P]]
[[Category: Lan, P]]
[[Category: Lei, M]]
[[Category: Tan, M]]
[[Category: Wu, J]]
[[Category: Hydrolase-rna complex]]
[[Category: Ribonuclease p]]
[[Category: Rna-protein complex]]