View source for 4d5n


==Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state==
<SX load='4d5n' size='340' side='right' viewer='molstar' caption='[[4d5n]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4d5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4D5N FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4d5l|4d5l]], [[4d5y|4d5y]], [[4d61|4d61]], [[4d66|4d66]], [[4d67|4d67]], [[4d68|4d68]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4d5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5n OCA], [http://pdbe.org/4d5n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d5n RCSB], [http://www.ebi.ac.uk/pdbsum/4d5n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d5n ProSAT]</span></td></tr>
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== Function ==
[[http://www.uniprot.org/uniprot/ERF1_HUMAN ERF1_HUMAN]] Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.<ref>PMID:7990965</ref>  
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== Publication Abstract from PubMed ==
The cricket paralysis virus (CrPV) uses an internal ribosomal entry site (IRES) to hijack the ribosome. In a remarkable RNA-based mechanism involving neither initiation factor nor initiator tRNA, the CrPV IRES jumpstarts translation in the elongation phase from the ribosomal A site. Here, we present cryoelectron microscopy (cryo-EM) maps of 80SCrPV-STOPeRF1eRF3GMPPNP and 80SCrPV-STOPeRF1 complexes, revealing a previously unseen binding state of the IRES and directly rationalizing that an eEF2-dependent translocation of the IRES is required to allow the first A-site occupation. During this unusual translocation event, the IRES undergoes a pronounced conformational change to a more stretched conformation. At the same time, our structural analysis provides information about the binding modes of eRF1eRF3GMPPNP and eRF1 in a minimal system. It shows that neither eRF3 nor ABCE1 are required for the active conformation of eRF1 at the intersection between eukaryotic termination and recycling.

Cryo-EM of Ribosomal 80S Complexes with Termination Factors Reveals the Translocated Cricket Paralysis Virus IRES.,Muhs M, Hilal T, Mielke T, Skabkin MA, Sanbonmatsu KY, Pestova TV, Spahn CM Mol Cell. 2015 Feb 5;57(3):422-432. doi: 10.1016/j.molcel.2014.12.016. Epub 2015 , Jan 15. PMID:25601755<ref>PMID:25601755</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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[[Category: Human]]
[[Category: Large Structures]]
[[Category: Hilal, T]]
[[Category: Mielke, T]]
[[Category: Muhs, M]]
[[Category: Pestova, T V]]
[[Category: Sanbonmatsu, K Y]]
[[Category: Skabkin, M A]]
[[Category: Spahn, C M.T]]
[[Category: Crpv ire]]
[[Category: Release factor]]
[[Category: Ribosome]]
[[Category: Ribosome-rna complex]]
[[Category: Termination]]