View source for 5dcq


==Crystal structure of bacterial adhesin, FNE from Streptococcus equi spp. equi.==
<StructureSection load='5dcq' size='340' side='right'caption='[[5dcq]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5dcq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences] and [http://en.wikipedia.org/wiki/Streptococcus_equi_subsp._equi Streptococcus equi subsp. equi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DCQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DCQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4pfg|4pfg]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fne ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=148942 Streptococcus equi subsp. equi])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5dcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dcq OCA], [http://pdbe.org/5dcq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dcq RCSB], [http://www.ebi.ac.uk/pdbsum/5dcq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dcq ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Streptococcus equi is a horse pathogen belonging to Lancefield group C. Infection by S. equi ssp. equi causes strangles, a serious and highly contagious disease of the upper respiratory tract. S. equi ssp. equi secretes a fibronectin (Fn)-binding protein, FNE, that does not contain cell wall-anchoring motifs. FNE binds to the gelatin-binding domain (GBD) of Fn, composed of the motifs (6) FI (12) FII (789) FI . FNE lacks the canonical Fn-binding peptide repeats observed in many microbial surface components recognizing adhesive matrix molecules. We found that the interaction between FNE and the human GBD is mediated by the binding of the disordered C-terminal region (residues 208-262) of FNE to the (789) FI GBD subfragment. The crystal structure of FNE showed that it is similar to the minor pilus protein Spy0125 of Streptococcus pyogenes, found at the end of pilus polymers and responsible for adhesion. FNE and Spy0125 both have a superimposable internal thioester bond between highly conserved Cys and Gln residues. Small-angle X-ray scattering of the FNE-(789) FI complex provided a model that aligns the C-terminal peptide of FNE with the E-strands of the FI domains, adopting the beta-zipper extension model observed in previous structures of microbial surface components recognizing adhesive matrix molecule adhesion peptides bound to FI domains.

Structural and functional analysis of the fibronectin-binding protein FNE from Streptococcus equi spp. equi.,Tiouajni M, Durand D, Blondeau K, Graille M, Urvoas A, Valerio-Lepiniec M, Guellouz A, Aumont-Nicaise M, Minard P, van Tilbeurgh H FEBS J. 2014 Dec;281(24):5513-31. doi: 10.1111/febs.13092. Epub 2014 Nov 4. PMID:25290767<ref>PMID:25290767</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus equi subsp. equi]]
[[Category: Synthetic construct sequences]]
[[Category: Graille, M]]
[[Category: Tilbeurgh, H van]]
[[Category: Tiouajni, M]]
[[Category: Adhesin]]
[[Category: Artificial repeat protein]]
[[Category: Complex]]
[[Category: Extracellular matrix]]
[[Category: Pilus]]
[[Category: Structural protein]]
[[Category: Thioester bond]]