View source for 6vqx


==Type I-F CRISPR-Csy complex with its inhibitor AcrF6==
<StructureSection load='6vqx' size='340' side='right'caption='[[6vqx]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6vqx]] is a 11 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6VQX FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6vqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vqx OCA], [http://pdbe.org/6vqx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vqx RCSB], [http://www.ebi.ac.uk/pdbsum/6vqx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vqx ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/CAS6_PSEAB CAS6_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:20829488</ref> <ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Chiu, W]]
[[Category: Huang, Z]]
[[Category: Li, S]]
[[Category: Pintilie, G]]
[[Category: Zhang, K]]
[[Category: Zhu, Y]]
[[Category: Acrf6]]
[[Category: Anti-crispr]]
[[Category: Crispr]]
[[Category: Csy]]
[[Category: Rna binding protein-rna-inhibitor complex]]
[[Category: Type i-f]]