View source for 4aoe


==Biomphalaria glabrata Acetylcholine-binding protein type 2 (BgAChBP2)==
<SX load='4aoe' size='340' side='right' viewer='molstar' caption='[[4aoe]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4aoe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Biomphalaria_glabrata Biomphalaria glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AOE FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aod|4aod]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aoe OCA], [http://pdbe.org/4aoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4aoe RCSB], [http://www.ebi.ac.uk/pdbsum/4aoe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4aoe ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Nicotinic acetylcholine receptors (nAChR) play important neurophysiological roles and are of considerable medical relevance. They have been studied extensively, greatly facilitated by the gastropod acetylcholine-binding proteins (AChBP) which represent soluble structural and functional homologues of the ligand-binding domain of nAChR. All these proteins are ring-like pentamers. Here we report that AChBP exists in the hemolymph of the planorbid snail Biomphalaria glabrata (vector of the schistosomiasis parasite) as a regular pentagonal dodecahedron, 22 nm in diameter (12 pentamers, 60 active sites). We sequenced and recombinantly expressed two approximately 25 kDa polypeptides (BgAChBP1 and BgAChBP2) with a specific active site, N-glycan site and disulfide bridge variation. We also provide the exon/intron structures. Recombinant BgAChBP1 formed pentamers and dodecahedra, recombinant BgAChBP2 formed pentamers and probably disulfide-bridged di-pentamers, but not dodecahedra. Three-dimensional electron cryo-microscopy (3D-EM) yielded a 3D reconstruction of the dodecahedron with a resolution of 6 A. Homology models of the pentamers docked to the 6 A structure revealed opportunities for chemical bonding at the inter-pentamer interfaces. Definition of the ligand-binding pocket and the gating C-loop in the 6 A structure suggests that 3D-EM might lead to the identification of functional states in the BgAChBP dodecahedron.

Acetylcholine-Binding Protein in the Hemolymph of the Planorbid Snail Biomphalaria glabrata Is a Pentagonal Dodecahedron (60 Subunits).,Saur M, Moeller V, Kapetanopoulos K, Braukmann S, Gebauer W, Tenzer S, Markl J PLoS One. 2012;7(8):e43685. Epub 2012 Aug 20. PMID:22916297<ref>PMID:22916297</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4aoe" style="background-color:#fffaf0;"></div>

==See Also==
*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Biomphalaria glabrata]]
[[Category: Large Structures]]
[[Category: Braukmann, S]]
[[Category: Gebauer, W]]
[[Category: Kapetanopoulos, K]]
[[Category: Markl, J]]
[[Category: Moeller, V]]
[[Category: Saur, M]]
[[Category: Tenzer, S]]
[[Category: Acetylcholine]]
[[Category: Acetylcholine receptor]]
[[Category: Acetylcholine-binding protein]]
[[Category: Achbp]]
[[Category: Achr]]
[[Category: Bilharziosis]]
[[Category: Cys-loop receptor]]
[[Category: Lgic]]
[[Category: Ligand gated ion channel]]
[[Category: Myasthenia gravi]]
[[Category: Nicotinic]]
[[Category: Pentagonal dodecahedron]]
[[Category: Schistosoma mansoni]]