View source for 6t12


==Crystal structure of YTHDC1 with fragment 30 (DHU_DC1_220)==
<StructureSection load='6t12' size='340' side='right'caption='[[6t12]], [[Resolution|resolution]] 1.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6t12]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T12 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6T12 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=M78:~{N},2,3-trimethyl-1~{H}-indole-5-carboxamide'>M78</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6t12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t12 OCA], [http://pdbe.org/6t12 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t12 RCSB], [http://www.ebi.ac.uk/pdbsum/6t12 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t12 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/YTDC1_HUMAN YTDC1_HUMAN]] RNA-binding protein that regulates alternative splice site selection.<ref>PMID:20167602</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report a crystallographic analysis of small-molecule ligands of the human YTHDC1 domain that recognizes N6-methylated adenine (m(6)A) in RNA. The 30 binders are fragments (molecular weight &lt; 300 g mol(-1)) that represent 10 different chemotypes identified by virtual screening. Despite the structural disorder of the binding site loop (residues 429-439), most of the 30 fragments emulate the two main interactions of the -NHCH3 group of m(6)A. These interactions are the hydrogen bond to the backbone carbonyl of Ser378 and the van der Waals contacts with the tryptophan cage. Different chemical groups are involved in the conserved binding motifs. Some of the fragments show favorable ligand efficiency for YTHDC1 and selectivity against other m(6)A reader domains. The structural information is useful for the design of modulators of m(6)A recognition by YTHDC1.

Selectively Disrupting m(6)A-Dependent Protein-RNA Interactions with Fragments.,Bedi RK, Huang D, Wiedmer L, Li Y, Dolbois A, Wojdyla JA, Sharpe ME, Caflisch A, Sledz P ACS Chem Biol. 2020 Mar 2. doi: 10.1021/acschembio.9b00894. PMID:32101404<ref>PMID:32101404</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6t12" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Bedi, R K]]
[[Category: Caflisch, A]]
[[Category: Huang, D]]
[[Category: Sledz, P]]
[[Category: Complex]]
[[Category: Epitranscriptomic]]
[[Category: Fragment]]
[[Category: Rna binding protein]]
[[Category: Ythdc1]]