View source for 6e06


==Crystal structure of Mycobacterium tuberculosis dethiobiotin synthetase in complex with cytidine triphosphate solved by precipitant-ligand exchange (crystals grown in citrate precipitant)==
<StructureSection load='6e06' size='340' side='right'caption='[[6e06]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6e06]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E06 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E06 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wop|4wop]], [[6cve|6cve]], [[6cvu|6cvu]], [[6cvv|6cvv]], [[6cze|6cze]], [[6czb|6czb]], [[6czc|6czc]], [[6cvf|6cvf]], [[6czd|6czd]], [[6e05|6e05]], [[3fgn|3fgn]], [[3fmf|3fmf]], [[3fpa|3fpa]], [[3fmi|3fmi]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bioD, Rv1570, MTCY336.33c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dethiobiotin_synthase Dethiobiotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.3 6.3.3.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e06 OCA], [http://pdbe.org/6e06 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e06 RCSB], [http://www.ebi.ac.uk/pdbsum/6e06 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e06 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/BIOD_MYCTU BIOD_MYCTU]] Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring.<ref>PMID:20565114</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dethiobiotin synthetase from Mycobacterium tuberculosis (MtDTBS) is a promising antituberculosis drug target. Small-molecule inhibitors that target MtDTBS provide a route towards new therapeutics for the treatment of antibiotic-resistant tuberculosis. Adenosine diphosphate (ADP) is an inhibitor of MtDTBS; however, structural studies into its mechanism of inhibition have been unsuccessful owing to competitive binding to the enzyme by crystallographic precipitants such as citrate and sulfate. Here, a crystallographic technique termed precipitant-ligand exchange has been developed to exchange protein-bound precipitants with ligands of interest. Proof of concept for the exchange method was demonstrated using cytidine triphosphate (CTP), which adopted the same binding mechanism as that obtained with traditional crystal-soaking techniques. Precipitant-ligand exchange also yielded the previously intractable structure of MtDTBS in complex with ADP solved to 2.4 A resolution. This result demonstrates the utility of precipitant-ligand exchange, which may be widely applicable to protein crystallography.

Precipitant-ligand exchange technique reveals the ADP binding mode in Mycobacterium tuberculosis dethiobiotin synthetase.,Thompson AP, Wegener KL, Booker GW, Polyak SW, Bruning JB Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):965-972. doi:, 10.1107/S2059798318010136. Epub 2018 Oct 2. PMID:30289406<ref>PMID:30289406</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6e06" style="background-color:#fffaf0;"></div>

==See Also==
*[[Dethiobiotin synthetase 3D structures|Dethiobiotin synthetase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dethiobiotin synthase]]
[[Category: Large Structures]]
[[Category: Myctu]]
[[Category: Bruning, J B]]
[[Category: Polyak, S W]]
[[Category: Thompson, A P]]
[[Category: Wegener, K L]]
[[Category: Enzyme]]
[[Category: Nucleotide triphosphate binding]]
[[Category: Synthetase]]
[[Category: Transferase]]