View source for 5ioo


==Accommodation of massive sequence variation in Nanoarchaeota by the C-type lectin fold==
<StructureSection load='5ioo' size='340' side='right'caption='[[5ioo]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ioo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nanoarchaeota_archaeon_jgi_otu-1 Nanoarchaeota archaeon jgi otu-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IOO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ioo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ioo OCA], [http://pdbe.org/5ioo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ioo RCSB], [http://www.ebi.ac.uk/pdbsum/5ioo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ioo ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
BACKGROUND: Diversity-generating retroelements (DGRs) provide organisms with a unique means for adaptation to a dynamic environment through massive protein sequence variation. The potential scope of this variation exceeds that of the vertebrate adaptive immune system. DGRs were known to exist only in viruses and bacteria until their recent discovery in archaea belonging to the 'microbial dark matter', specifically in organisms closely related to Nanoarchaeota. However, Nanoarchaeota DGR variable proteins were unassignable to known protein folds and apparently unrelated to characterized DGR variable proteins. RESULTS: To address the issue of how Nanoarchaeota DGR variable proteins accommodate massive sequence variation, we determined the 2.52 A resolution limit crystal structure of one such protein, AvpA, which revealed a C-type lectin (CLec)-fold that organizes a putative ligand-binding site that is capable of accommodating 10(13) sequences. This fold is surprisingly reminiscent of the CLec-folds of viral and bacterial DGR variable protein, but differs sufficiently to define a new CLec-fold subclass, which is consistent with early divergence between bacterial and archaeal DGRs. The structure also enabled identification of a group of AvpA-like proteins in multiple putative DGRs from uncultivated archaea. These variable proteins may aid Nanoarchaeota and these uncultivated archaea in symbiotic relationships. CONCLUSIONS: Our results have uncovered the widespread conservation of the CLec-fold in viruses, bacteria, and archaea for accommodating massive sequence variation. In addition, to our knowledge, this is the first report of an archaeal CLec-fold protein.

Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements.,Handa S, Paul BG, Miller JF, Valentine DL, Ghosh P BMC Struct Biol. 2016 Aug 31;16(1):13. doi: 10.1186/s12900-016-0064-6. PMID:27578274<ref>PMID:27578274</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5ioo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nanoarchaeota archaeon jgi otu-1]]
[[Category: Ghosh, P]]
[[Category: Handa, S]]
[[Category: Miller, J]]
[[Category: Paul, B]]
[[Category: Valentine, D]]
[[Category: Archaea]]
[[Category: Clec fold]]
[[Category: Massive protein diversification]]
[[Category: Sugar binding protein]]
[[Category: Unknown function]]