View source for 5uvi


==Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation==
<StructureSection load='5uvi' size='340' side='right'caption='[[5uvi]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5uvi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UVI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uvj|5uvj]], [[5uvk|5uvk]], [[5uvl|5uvl]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ADORA2A, ADORA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uvi OCA], [http://pdbe.org/5uvi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uvi RCSB], [http://www.ebi.ac.uk/pdbsum/5uvi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uvi ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/AA2AR_HUMAN AA2AR_HUMAN]] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial millisecond crystallography (SMX). As a result, the number of SMX experiments is growing rapidly, with a dozen experiments reported so far. Here, the first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported. Microcrystals (5-20 microm) of a wide variety of proteins, including lysozyme, thaumatin, phycocyanin, the human A2A adenosine receptor (A2AAR), the soluble fragment of the membrane lipoprotein Flpp3 and proteinase K, were screened. Crystals suspended in lipidic cubic phase (LCP) or a high-molecular-weight poly(ethylene oxide) (PEO; molecular weight 8 000 000) were delivered to the beam using a high-viscosity injector. In-house data-reduction (hit-finding) software developed at APS as well as the SFX data-reduction and analysis software suites Cheetah and CrystFEL enabled efficient on-site SMX data monitoring, reduction and processing. Complete data sets were collected for A2AAR, phycocyanin, Flpp3, proteinase K and lysozyme, and the structures of A2AAR, phycocyanin, proteinase K and lysozyme were determined at 3.2, 3.1, 2.65 and 2.05 A resolution, respectively. The data demonstrate the feasibility of serial millisecond crystallography from 5-20 microm crystals using a high-viscosity injector at APS. The resolution of the crystal structures obtained in this study was dictated by the current flux density and crystal size, but upcoming developments in beamline optics and the planned APS-U upgrade will increase the intensity by two orders of magnitude. These developments will enable structure determination from smaller and/or weakly diffracting microcrystals.

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation.,Martin-Garcia JM, Conrad CE, Nelson G, Stander N, Zatsepin NA, Zook J, Zhu L, Geiger J, Chun E, Kissick D, Hilgart MC, Ogata C, Ishchenko A, Nagaratnam N, Roy-Chowdhury S, Coe J, Subramanian G, Schaffer A, James D, Ketwala G, Venugopalan N, Xu S, Corcoran S, Ferguson D, Weierstall U, Spence JCH, Cherezov V, Fromme P, Fischetti RF, Liu W IUCrJ. 2017 May 24;4(Pt 4):439-454. doi: 10.1107/S205225251700570X. eCollection, 2017 Jul 1. PMID:28875031<ref>PMID:28875031</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5uvi" style="background-color:#fffaf0;"></div>

==See Also==
*[[Adenosine A2A receptor 3D structures|Adenosine A2A receptor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Large Structures]]
[[Category: Cherezov, V]]
[[Category: Chun, E]]
[[Category: Coe, J]]
[[Category: Conrad, C E]]
[[Category: Corcoran, S]]
[[Category: Ferguson, D]]
[[Category: Fischetti, R F]]
[[Category: Fromme, P]]
[[Category: Geiger, J]]
[[Category: Hilgart, M C]]
[[Category: Ishchenko, A]]
[[Category: James, D]]
[[Category: Ketawala, G]]
[[Category: Kissick, D]]
[[Category: Liu, W]]
[[Category: Martin-Garcia, J M]]
[[Category: Nagaratnam, N]]
[[Category: Nelson, G]]
[[Category: Ogata, C]]
[[Category: Roy-Chowdhury, S]]
[[Category: Schaffer, A]]
[[Category: Spence, J C.H]]
[[Category: Stander, N]]
[[Category: Subramanian, G]]
[[Category: Venugopalan, N]]
[[Category: Weierstall, U]]
[[Category: Xu, S]]
[[Category: Zatsepin, N A]]
[[Category: Zhu, L]]
[[Category: Zook, J]]
[[Category: Gpcr]]
[[Category: Membrane protein]]