View source for 3v2w


==Crystal Structure of a Lipid G protein-Coupled Receptor at 3.35A==
<StructureSection load='3v2w' size='340' side='right'caption='[[3v2w]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3v2w]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V2W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ML5:{(3R)-3-AMINO-4-[(3-HEXYLPHENYL)AMINO]-4-OXOBUTYL}PHOSPHONIC+ACID'>ML5</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3v2y|3v2y]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2w OCA], [http://pdbe.org/3v2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v2w RCSB], [http://www.ebi.ac.uk/pdbsum/3v2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v2w ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/S1PR1_HUMAN S1PR1_HUMAN]] G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.<ref>PMID:10982820</ref> <ref>PMID:11230698</ref> <ref>PMID:11583630</ref> <ref>PMID:11604399</ref> <ref>PMID:19286607</ref> <ref>PMID:22344443</ref> <ref>PMID:8626678</ref> <ref>PMID:9488656</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The lyso-phospholipid sphingosine 1-phosphate modulates lymphocyte trafficking, endothelial development and integrity, heart rate, and vascular tone and maturation by activating G protein-coupled sphingosine 1-phosphate receptors. Here, we present the crystal structure of the sphingosine 1-phosphate receptor 1 fused to T4-lysozyme (S1P(1)-T4L) in complex with an antagonist sphingolipid mimic. Extracellular access to the binding pocket is occluded by the amino terminus and extracellular loops of the receptor. Access is gained by ligands entering laterally between helices I and VII within the transmembrane region of the receptor. This structure, along with mutagenesis, agonist structure-activity relationship data, and modeling, provides a detailed view of the molecular recognition and requirement for hydrophobic volume that activates S1P(1), resulting in the modulation of immune and stromal cell responses.

Crystal structure of a lipid G protein-coupled receptor.,Hanson MA, Roth CB, Jo E, Griffith MT, Scott FL, Reinhart G, Desale H, Clemons B, Cahalan SM, Schuerer SC, Sanna MG, Han GW, Kuhn P, Rosen H, Stevens RC Science. 2012 Feb 17;335(6070):851-5. PMID:22344443<ref>PMID:22344443</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3v2w" style="background-color:#fffaf0;"></div>

==See Also==
*[[G protein-coupled receptor|G protein-coupled receptor]]
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lysozyme]]
[[Category: Cahalan, S M]]
[[Category: Clemons, B]]
[[Category: Desale, H]]
[[Category: GPCR, GPCR Network]]
[[Category: Griffith, M T]]
[[Category: Han, G W]]
[[Category: Hanson, M A]]
[[Category: Jo, E]]
[[Category: Kuhn, P]]
[[Category: Reinhart, G]]
[[Category: Rosen, H]]
[[Category: Roth, C B]]
[[Category: Sanna, M G]]
[[Category: Schuerer, S C]]
[[Category: Scott, F L]]
[[Category: Stevens, R C]]
[[Category: Autoimmunity]]
[[Category: Edg receptor]]
[[Category: G protein coupled receptor]]
[[Category: Gpcr]]
[[Category: Gpcr network]]
[[Category: Hydrolase]]
[[Category: Lipid receptor]]
[[Category: Membrane]]
[[Category: Membrane protein]]
[[Category: Multiple sclerosis]]
[[Category: Psi-biology]]
[[Category: Sphingosine]]
[[Category: Structural genomic]]