View source for 6itq


==Crystal structure of cortisol complexed with its nanobody at pH 10.5==
<StructureSection load='6itq' size='340' side='right'caption='[[6itq]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6itq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ITQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ITQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HCY:(11ALPHA,14BETA)-11,17,21-TRIHYDROXYPREGN-4-ENE-3,20-DIONE'>HCY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6itq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6itq OCA], [http://pdbe.org/6itq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6itq RCSB], [http://www.ebi.ac.uk/pdbsum/6itq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6itq ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To date, few structural models of VHH antibody binding to low molecular weight haptens have been reported. Here, we report the crystal structure of cortisol binding to its VHH antibody NbCor at pH 3.5 and 10.5. Cortisol binds to NbCor mainly by burying itself under the tunnel formed by the complementarity determining region 1 (CDR1) of NbCor. The affinity of NbCor binding to cortisol and similar compounds was also verified by a microscale thermophoresis assay. Combining our findings with several previously reported structures of hapten-VHH antibody complexes, we propose that VHH antibodies exhibit a special mechanism of binding small haptens by encapsulating them in a tunnel formed by CDR1. Our findings provide useful structural information for the further development and optimization of hapten-specific VHH antibodies.

Structural insights into the mechanism of single domain VHH antibody binding to cortisol.,Ding L, Wang Z, Zhong P, Jiang H, Zhao Z, Zhang Y, Ren Z, Ding Y FEBS Lett. 2019 Jun;593(11):1248-1256. doi: 10.1002/1873-3468.13398. Epub 2019, May 20. PMID:31049949<ref>PMID:31049949</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6itq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ding, L L]]
[[Category: Ding, Y]]
[[Category: Wang, Z Y]]
[[Category: Zhong, P Y]]
[[Category: Camelid antibody]]
[[Category: Complex]]
[[Category: Cortisol]]
[[Category: Immune system]]
[[Category: Nanobody]]