View source for 5and


==Crystal structure of CDK2 in complex with 2-imidazol-1-yl-1H- benzimidazole processed with the CrystalDirect automated mounting and cryo-cooling technology==
<StructureSection load='5and' size='340' side='right'caption='[[5and]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5and]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AND OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AND FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5JE:2-IMIDAZOL-1-YL-1H-BENZIMIDAZOLE'>5JE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5amw|5amw]], [[5amx|5amx]], [[5amy|5amy]], [[5amz|5amz]], [[5an2|5an2]], [[5an4|5an4]], [[5ane|5ane]], [[5ang|5ang]], [[5ani|5ani]], [[5anj|5anj]], [[5ank|5ank]], [[5anl|5anl]], [[5ano|5ano]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5and FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5and OCA], [http://pdbe.org/5and PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5and RCSB], [http://www.ebi.ac.uk/pdbsum/5and PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5and ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting and processing largely depend on manual operations. Here, a series of new methods are presented based on the use of a low X-ray-background film as a crystallization support and a photoablation laser that enable the automation of major operations required for the preparation of crystals for X-ray diffraction experiments. In this approach, the controlled removal of the mother liquor before crystal mounting simplifies the cryocooling process, in many cases eliminating the use of cryoprotectant agents, while crystal-soaking experiments are performed through diffusion, precluding the need for repeated sample-recovery and transfer operations. Moreover, the high-precision laser enables new mounting strategies that are not accessible through other methods. This approach bridges an important gap in automation and can contribute to expanding the capabilities of modern macromolecular crystallography facilities.

Automated harvesting and processing of protein crystals through laser photoablation.,Zander U, Hoffmann G, Cornaciu I, Marquette JP, Papp G, Landret C, Seroul G, Sinoir J, Rower M, Felisaz F, Rodriguez-Puente S, Mariaule V, Murphy P, Mathieu M, Cipriani F, Marquez JA Acta Crystallogr D Struct Biol. 2016 Apr 1;72(Pt 4):454-66. doi:, 10.1107/S2059798316000954. Epub 2016 Mar 24. PMID:27050125<ref>PMID:27050125</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5and" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cyclin-dependent kinase|Cyclin-dependent kinase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Cipriani, F]]
[[Category: Cornaciu, I]]
[[Category: Hoffmann, G]]
[[Category: Marquette, J P]]
[[Category: Marquez, J A]]
[[Category: Mathieu, M]]
[[Category: Zander, U]]
[[Category: Automated cryo-cooling]]
[[Category: Automated crystal harvesting]]
[[Category: Crystaldirect]]
[[Category: Inhibitor]]
[[Category: Protein kinase]]
[[Category: Transferase]]