View source for 4p0r


==human Mus81-Eme1-3'flap DNA complex==
<StructureSection load='4p0r' size='340' side='right'caption='[[4p0r]], [[Resolution|resolution]] 6.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4p0r]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P0R FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4p0p|4p0p]], [[4p0q|4p0q]], [[4p0s|4p0s]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), EME1, MMS4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p0r OCA], [http://pdbe.org/4p0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p0r RCSB], [http://www.ebi.ac.uk/pdbsum/4p0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p0r ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/MUS81_HUMAN MUS81_HUMAN]] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:11741546</ref> <ref>PMID:12374758</ref> <ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:15805243</ref> <ref>PMID:17289582</ref> <ref>PMID:19595721</ref> <ref>PMID:19596235</ref>  [[http://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Mus81-Eme1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. To explain the molecular basis of 3' flap substrate recognition and cleavage mechanism by Mus81-Eme1, we determined crystal structures of human Mus81-Eme1 bound to various flap DNA substrates. Mus81-Eme1 undergoes gross substrate-induced conformational changes that reveal two key features: (i) a hydrophobic wedge of Mus81 that separates pre- and post-nick duplex DNA and (ii) a "5' end binding pocket" that hosts the 5' nicked end of post-nick DNA. These features are crucial for comprehensive protein-DNA interaction, sharp bending of the 3' flap DNA substrate, and incision strand placement at the active site. While Mus81-Eme1 unexpectedly shares several common features with members of the 5' flap nuclease family, the combined structural, biochemical, and biophysical analyses explain why Mus81-Eme1 preferentially cleaves 3' flap DNA substrates with 5' nicked ends.

Crystal structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates.,Gwon GH, Jo A, Baek K, Jin KS, Fu Y, Lee JB, Kim Y, Cho Y EMBO J. 2014 May 2;33(9):1061-72. doi: 10.1002/embj.201487820. Epub 2014 Apr 14. PMID:24733841<ref>PMID:24733841</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4p0r" style="background-color:#fffaf0;"></div>

==See Also==
*[[Endonuclease|Endonuclease]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Baek, K]]
[[Category: Cho, Y]]
[[Category: Gwon, G H]]
[[Category: Hydrolase-dna complex]]
[[Category: Resolvase]]