View source for 4csf


==Structural insights into Toscana virus RNA encapsidation==
<StructureSection load='4csf' size='340' side='right'caption='[[4csf]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4csf]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Tos Tos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CSF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CSF FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4csg|4csg]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4csf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4csf OCA], [http://pdbe.org/4csf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4csf RCSB], [http://www.ebi.ac.uk/pdbsum/4csf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4csf ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses.

Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein.,Olal D, Dick A, Woods VL Jr, Liu T, Li S, Devignot S, Weber F, Saphire EO, Daumke O Nucleic Acids Res. 2014 Mar 31. PMID:24688060<ref>PMID:24688060</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4csf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nucleoprotein|Nucleoprotein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tos]]
[[Category: Daumke, O]]
[[Category: Olal, D]]
[[Category: Hexameric]]
[[Category: Infectious]]
[[Category: Nucleocapsid]]
[[Category: Nucleoprotein]]
[[Category: Viral protein]]
[[Category: Viral protein-rna complex]]