View source for 4a68


==Mutations in the neighbourhood of CotA-laccase trinuclear site: D116N mutant==
<StructureSection load='4a68' size='340' side='right'caption='[[4a68]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4a68]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A68 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1uvw|1uvw]], [[1of0|1of0]], [[1w6w|1w6w]], [[2x88|2x88]], [[4a67|4a67]], [[4a66|4a66]], [[1w8e|1w8e]], [[1w6l|1w6l]], [[1gsk|1gsk]], [[2bhf|2bhf]], [[2x87|2x87]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a68 OCA], [http://pdbe.org/4a68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a68 RCSB], [http://www.ebi.ac.uk/pdbsum/4a68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a68 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis. 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Multi-copper oxidases constitute a family of proteins that are capable of coupling the one-electron oxidation of four substrate equivalents to the four-electron reduction of dioxygen to two molecules of water. The main catalytic stages occurring during the process have already been identified, but several questions remain, including the nature of the protonation events that take place during the reductive cleavage of dioxygen to water. The presence of a structurally conserved acidic residue (Glu498 in CotA laccase from Bacillus subtilis) at the dioxygen-entrance channel has been reported to play a decisive role in the protonation mechanisms, channelling protons during the reduction process and stabilizing the site as a whole. A second acidic residue that is sequentially conserved in multi-copper oxidases and sited within the exit channel (Asp116 in CotA) has also been identified as being important in the protonation process. In this study, CotA laccase has been used as a model system to assess the role of Asp116 in the reduction process of dioxygen to water. The crystal structures of three distinct mutants, D116E, D116N and D116A, produced by site-saturation mutagenesis have been determined. In addition, theoretical calculations have provided further support for a role of this residue in the protonation events.

The role of Asp116 in the reductive cleavage of dioxygen to water in CotA laccase: assistance during the proton-transfer mechanism.,Silva CS, Damas JM, Chen Z, Brissos V, Martins LO, Soares CM, Lindley PF, Bento I Acta Crystallogr D Biol Crystallogr. 2012 Feb;68(Pt 2):186-93. Epub 2012 Jan 17. PMID:22281748<ref>PMID:22281748</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4a68" style="background-color:#fffaf0;"></div>

==See Also==
*[[CotA laccase|CotA laccase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Vibrio subtilis ehrenberg 1835]]
[[Category: Laccase]]
[[Category: Bento, I]]
[[Category: Lindley, P F]]
[[Category: Silva, C S]]
[[Category: Dioxygen reduction]]
[[Category: Multi-copper oxidase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase activity]]
[[Category: Trinuclear cluster]]