View source for 6he5


==20S core particle of PAN-proteasomes==
<StructureSection load='6he5' size='340' side='right' caption='[[6he5]], [[Resolution|resolution]] 4.12&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6he5]] is a 20 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HE5 FirstGlance]. <br>
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6he5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6he5 OCA], [http://pdbe.org/6he5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6he5 RCSB], [http://www.ebi.ac.uk/pdbsum/6he5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6he5 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PSA_ARCFU PSA_ARCFU]] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation (By similarity).[HAMAP-Rule:MF_00289_A] [[http://www.uniprot.org/uniprot/PAN_ARCFU PAN_ARCFU]] ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates (Probable).<ref>PMID:19481487</ref>  [[http://www.uniprot.org/uniprot/PSB_ARCFU PSB_ARCFU]] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation (By similarity).[HAMAP-Rule:MF_02113_A] 
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== Publication Abstract from PubMed ==
Proteasomes occur in all three domains of life, and are the principal molecular machines for the regulated degradation of intracellular proteins. They play key roles in the maintenance of protein homeostasis, and control vital cellular processes. While the eukaryotic 26S proteasome is extensively characterized, its putative evolutionary precursor, the archaeal proteasome, remains poorly understood. The primordial archaeal proteasome consists of a 20S proteolytic core particle (CP), and an AAA-ATPase module. This minimal complex degrades protein unassisted by non-ATPase subunits that are present in a 26S proteasome regulatory particle (RP). Using cryo-EM single-particle analysis, we determined structures of the archaeal CP in complex with the AAA-ATPase PAN (proteasome-activating nucleotidase). Five conformational states were identified, elucidating the functional cycle of PAN, and its interaction with the CP. Coexisting nucleotide states, and correlated intersubunit signaling features, coordinate rotation of the PAN-ATPase staircase, and allosterically regulate N-domain motions and CP gate opening. These findings reveal the structural basis for a sequential around-the-ring ATPase cycle, which is likely conserved in AAA-ATPases.

Cryo-EM structures of the archaeal PAN-proteasome reveal an around-the-ring ATPase cycle.,Majumder P, Rudack T, Beck F, Danev R, Pfeifer G, Nagy I, Baumeister W Proc Natl Acad Sci U S A. 2018 Dec 17. pii: 1817752116. doi:, 10.1073/pnas.1817752116. PMID:30559193<ref>PMID:30559193</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6he5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Proteasome endopeptidase complex]]
[[Category: Baumeister, W]]
[[Category: Beck, F]]
[[Category: Majumder, P]]
[[Category: Rudack, T]]
[[Category: Aaa-atpase]]
[[Category: Archaea]]
[[Category: Hydrolase]]
[[Category: Pan]]
[[Category: Proteasome]]