View source for 2z4u

[[Image:2z4u.jpg|left|200px]]

 {{Structure
|PDB= 2z4u |SIZE=350|CAPTION= <scene name='initialview01'>2z4u</scene>, resolution 1.1&Aring;
|SITE= <scene name='pdbsite=AC1:Edo+Binding+Site+For+Residue+A+501'>AC1</scene>
|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
|GENE= 
|DOMAIN=
|RELATEDENTRY=[[1qcg|1qcg]], [[2z53|2z53]], [[2qes|2QES]], [[2qet|2QET]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4u OCA], [http://www.ebi.ac.uk/pdbsum/2z4u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z4u RCSB]</span>
}}

'''Crystal structure of wild type PD-L4 from Phytolacca dioica leaves'''


==Overview==
The ribosome inactivating protein PD-L4 from Phytolacca dioica is a N-beta-glycosidase, probably involved in plant defence. The crystal structures of wild type PD-L4 and of the S211A PD-L4 mutant with significantly decreased catalytic activity were determined at atomic resolution. To determine the structural determinants for the reduced activity of S211A PD-L4, both forms have also been co-crystallized with adenine, the major product of PD-L4 catalytic reaction. In the structure of the S211A mutant, the cavity formed by the lack of the Ser hydroxyl group is filled by a water molecule; the insertion of this non-isosteric group leads to small albeit concerted changes in the tightly packed active site of the enzyme. These changes have been correlated to the different activity of the mutant enzyme. This work highlights the importance of atomic resolution studies for the deep understanding of enzymatic properties. Proteins 2008. (c) 2007 Wiley-Liss, Inc.

==About this Structure==
2Z4U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_dioica Phytolacca dioica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4U OCA]. 

==Reference==
Atomic resolution (1.1 A) structure of the ribosome-inactivating protein PD-L4 from Phytolacca dioica L. leaves., Ruggiero A, Chambery A, Maro AD, Parente A, Berisio R, Proteins. 2007 Oct 26;71(1):8-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17963235 17963235]
[[Category: Phytolacca dioica]]
[[Category: Single protein]]
[[Category: rRNA N-glycosylase]]
[[Category: Berisio, R.]]
[[Category: Ruggiero, A.]]
[[Category: crystallization]]
[[Category: hydrolase]]
[[Category: ribosome-inactivating protein]]
[[Category: x-ray]]

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