View source for 2w5t


==Structure-based mechanism of lipoteichoic acid synthesis by Staphylococcus aureus LtaS.==
<StructureSection load='2w5t' size='340' side='right' caption='[[2w5t]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2w5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W5T FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GP9:(2R)-2,3-DIHYDROXYPROPYL+PHOSPHATE'>GP9</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2w5q|2w5q]], [[2w5r|2w5r]], [[2w5s|2w5s]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5t OCA], [http://pdbe.org/2w5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w5t RCSB], [http://www.ebi.ac.uk/pdbsum/2w5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5t ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/LTAS_STAAW LTAS_STAAW]] Catalyzes the polymerization of lipoteichoic acid (LTA) polyglycerol phosphate, a reaction that presumably uses phosphatidylglycerol (PG) as substrate. Is required for staphylococcal growth and cell division process (By similarity). 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w5t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w5t ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococcus aureus synthesizes polyglycerol-phosphate lipoteichoic acid (LTA) from phosphatidylglycerol. LtaS, a predicted membrane protein with 5 N-terminal transmembrane helices followed by a large extracellular part (eLtaS), is required for staphylococcal growth and LTA synthesis. Here, we report the first crystal structure of the eLtaS domain at 1.2-A resolution and show that it assumes a sulfatase-like fold with an alpha/beta core and a C-terminal part composed of 4 anti-parallel beta-strands and a long alpha-helix. Overlaying eLtaS with sulfatase structures identified active site residues, which were confirmed by alanine substitution mutagenesis and in vivo enzyme function assays. The cocrystal structure with glycerol-phosphate and the coordination of a Mn(2+) cation allowed us to propose a reaction mechanism, whereby the active site threonine of LtaS functions as nucleophile for phosphatidylglycerol hydrolysis and formation of a covalent threonine-glycerolphosphate intermediate. These results will aid in the development of LtaS-specific inhibitors for S. aureus and many other Gram-positive pathogens.

Structure-based mechanism of lipoteichoic acid synthesis by Staphylococcus aureus LtaS.,Lu D, Wormann ME, Zhang X, Schneewind O, Grundling A, Freemont PS Proc Natl Acad Sci U S A. 2009 Jan 23. PMID:19168632<ref>PMID:19168632</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2w5t" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Freemont, P S]]
[[Category: Grundling, A]]
[[Category: Lu, D]]
[[Category: Schneewind, O]]
[[Category: Wormann, M E]]
[[Category: Zhang, X]]
[[Category: Cell membrane]]
[[Category: Cell wall biogenesis/degradation]]
[[Category: Glycerol-phosphate]]
[[Category: Lta]]
[[Category: Membrane]]
[[Category: Secreted]]
[[Category: Transferase]]
[[Category: Transmembrane]]