View source for 2px2

[[Image:2px2.jpg|left|200px]]

 {{Structure
|PDB= 2px2 |SIZE=350|CAPTION= <scene name='initialview01'>2px2</scene>, resolution 2.00&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span>
|GENE= NS5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11079 Murray Valley encephalitis virus])
|DOMAIN=
|RELATEDENTRY=[[2px4|2PX4]], [[2px5|2PX5]], [[2px8|2PX8]], [[2pxa|2PXA]], [[2pxc|2PXC]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2px2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2px2 OCA], [http://www.ebi.ac.uk/pdbsum/2px2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2px2 RCSB]</span>
}}

'''Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Monoclinic form 1)'''


==Overview==
We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

==About this Structure==
2PX2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Murray_valley_encephalitis_virus Murray valley encephalitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX2 OCA]. 

==Reference==
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17622627 17622627]
[[Category: Murray valley encephalitis virus]]
[[Category: RNA-directed RNA polymerase]]
[[Category: Single protein]]
[[Category: Alderton, D.]]
[[Category: Assenberg, R.]]
[[Category: Fuller, S D.]]
[[Category: Grimes, J M.]]
[[Category: Hurrelbrink, R J.]]
[[Category: OPPF, Oxford Protein Production Facility.]]
[[Category: Owens, R J.]]
[[Category: Ren, J.]]
[[Category: Stuart, D I.]]
[[Category: Verma, A.]]
[[Category: Walter, T S.]]
[[Category: methyltransferase]]
[[Category: murray valley encephalitis virus]]
[[Category: oppf]]
[[Category: oxford protein production facility]]
[[Category: sah]]
[[Category: structural genomic]]

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