View source for 2k0l


==NMR structure of the transmembrane domain of the Outer Membrane Protein A from Klebsiella pneumoniae in DHPC micelles.==
<StructureSection load='2k0l' size='340' side='right' caption='[[2k0l]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2k0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0L FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ompA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0l OCA], [http://pdbe.org/2k0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k0l RCSB], [http://www.ebi.ac.uk/pdbsum/2k0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k0l ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/OMPA_KLEPN OMPA_KLEPN]] Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes (By similarity). 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/2k0l_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k0l ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional structure of the outer membrane protein A from Klebsiella pneumoniae transmembrane domain was determined by NMR.This protein induces specific humoral and cytotoxic responses, and is a potent carrier protein. This is one of the largest integral membrane proteins(210 residues) for which nearly complete resonance assignment, including side chains, has been achieved so far. The methodology rested on the use of 900 MHz 3D and 4D TROSY experiments recorded on a uniformly 15N,13C,2H-labeled sample and on a perdeuterated methyl protonated sample. The structure was refined from 920 experimental constraints, giving an ensemble of 20 best structures with an r.m.s. deviation of 0.54 A for the main chain atoms in the core eight-stranded beta-barrel. The protein dynamics was assessed, in a residue-specific manner, by 1H-15N NOEs (pico- to nanosecond timescale), exchange broadening (millisecond to second) and 1H-2H chemical exchange (hour-weeks).

Solution state NMR structure and dynamics of KpOmpA, a 210 residue transmembrane domain possessing a high potential for immunological applications.,Renault M, Saurel O, Czaplicki J, Demange P, Gervais V, Lohr F, Reat V, Piotto M, Milon A J Mol Biol. 2009 Jan 9;385(1):117-30. Epub 2008 Oct 15. PMID:18952100<ref>PMID:18952100</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2k0l" style="background-color:#fffaf0;"></div>

==See Also==
*[[C-X-C motif chemokine|C-X-C motif chemokine]]
*[[Porin|Porin]]
*[[Stromal Derived Factor 1|Stromal Derived Factor 1]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gervais, V]]
[[Category: Lohr, F]]
[[Category: Milon, A]]
[[Category: Piotto, M]]
[[Category: Reat, V]]
[[Category: Renault, M]]
[[Category: Saurel, O]]
[[Category: Dhpc micelle]]
[[Category: Membrane protein]]
[[Category: Ompa]]
[[Category: Sidechain]]
[[Category: Trosy]]