View source for 2lef

[[Image:2lef.jpg|left|200px]]

 {{Structure
|PDB= 2lef |SIZE=350|CAPTION= <scene name='initialview01'>2lef</scene>
|SITE= 
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
|ACTIVITY= 
|GENE= LEF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lef OCA], [http://www.ebi.ac.uk/pdbsum/2lef PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2lef RCSB]</span>
}}

'''LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES'''


==Overview==
Lymphoid enhancer-binding factor (LEF-1) and the closely related T-cell factor 1 (TCF-1) are sequence-specific and cell-type-specific DNA-binding proteins that play important regulatory roles in organogenesis and thymocyte differentiation. LEF-1 participates in regulation of the enhancer associated with the T cell receptor (TCR)-alpha gene by inducing a sharp bend in the DNA and facilitating interactions between Ets-1, PEBP2-alpha, and ATF/CREB, transcription factors bound at sites flanking the LEF-1 site. It seems that LEF-1 plays an architectural role in the assembly and function of this regulatory nucleoprotein complex. LEF-1 recognizes a specific nucleotide sequence through a high-mobility-group (HMG) domain. Proteins containing HMG domains bind DNA in the minor groove, bend the double helix, and recognize four-way junctions and other irregular DNA structures. Here we report the solution structure of a complex of the LEF-1 HMG domain and adjacent basic region with its cognate DNA. The structure reveals the HMG domain bound in the widened minor groove of a markedly distorted and bent double helix. The basic region binds across the narrowed major groove and contributes to DNA recognition.

==About this Structure==
2LEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry 1LEF. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEF OCA]. 

==Reference==
Structural basis for DNA bending by the architectural transcription factor LEF-1., Love JJ, Li X, Case DA, Giese K, Grosschedl R, Wright PE, Nature. 1995 Aug 31;376(6543):791-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7651541 7651541]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Case, D A.]]
[[Category: Li, X.]]
[[Category: Love, J J.]]
[[Category: Wright, P E.]]
[[Category: complex (hmg domain/dna)]]
[[Category: dna bending]]
[[Category: dna binding]]
[[Category: hmg]]
[[Category: lef1]]
[[Category: tcr-a]]
[[Category: transcription factor]]

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