View source for 6clp


==1.16 A MicroED structure of GSNQNNF at 2.5 e- / A^2==
<StructureSection load='6clp' size='340' side='right' caption='[[6clp]], [[Resolution|resolution]] 1.16&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6clp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CLP FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6clp OCA], [http://pdbe.org/6clp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6clp RCSB], [http://www.ebi.ac.uk/pdbsum/6clp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6clp ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data.

Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.,Hattne J, Shi D, Glynn C, Zee CT, Gallagher-Jones M, Martynowycz MW, Rodriguez JA, Gonen T Structure. 2018 May 1;26(5):759-766.e4. doi: 10.1016/j.str.2018.03.021. Epub 2018, Apr 26. PMID:29706530<ref>PMID:29706530</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6clp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallagher-Jones, M]]
[[Category: Glynn, C]]
[[Category: Gonen, T]]
[[Category: Hattne, J]]
[[Category: Martynowycz, M W]]
[[Category: Rodriguez, J A]]
[[Category: Shi, D]]
[[Category: Zee, C T]]
[[Category: Amyloid fibril]]
[[Category: Prion]]
[[Category: Protein fibril]]
[[Category: Zinc binding]]