View source for 1w2b

[[Image:1w2b.gif|left|200px]]<br />
<applet load="1w2b" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1w2b, resolution 3.50&Aring;" />
'''TRIGGER FACTOR RIBOSOME BINDING DOMAIN IN COMPLEX WITH 50S'''<br />

==Overview==
During protein biosynthesis, nascent polypeptide chains that emerge from, the ribosomal exit tunnel encounter ribosome-associated chaperones, which, assist their folding to the native state. Here we present a 2.7 A crystal, structure of Escherichia coli trigger factor, the best-characterized, chaperone of this type, together with the structure of its, ribosome-binding domain in complex with the Haloarcula marismortui large, ribosomal subunit. Trigger factor adopts a unique conformation resembling, a crouching dragon with separated domains forming the amino-terminal, ribosome-binding 'tail', the peptidyl-prolyl isomerase 'head', the, carboxy-terminal 'arms' and connecting regions building up the 'back'., From its attachment point on the ribosome, trigger factor projects the, extended domains over the exit of the ribosomal tunnel, creating a, protected folding space where nascent polypeptides may be shielded from, proteases and aggregation. This study sheds new light on our understanding, of co-translational protein folding, and suggests an unexpected mechanism, of action for ribosome-associated chaperones.

==About this Structure==
1W2B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with MG, NA, K, CL and CD as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W2B OCA]. 

==Reference==
Trigger factor in complex with the ribosome forms a molecular cradle for nascent proteins., Ferbitz L, Maier T, Patzelt H, Bukau B, Deuerling E, Ban N, Nature. 2004 Sep 30;431(7008):590-6. Epub 2004 Aug 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15334087 15334087]
[[Category: Escherichia coli]]
[[Category: Haloarcula marismortui]]
[[Category: Protein complex]]
[[Category: Ban, N.]]
[[Category: Bukau, B.]]
[[Category: Deuerling, E.]]
[[Category: Ferbitz, L.]]
[[Category: Maier, T.]]
[[Category: Patzelt, H.]]
[[Category: CD]]
[[Category: CL]]
[[Category: K]]
[[Category: MG]]
[[Category: NA]]
[[Category: chaperone]]
[[Category: cotranslational folding]]
[[Category: nascent chain]]
[[Category: ribosomal protein]]
[[Category: ribosome]]
[[Category: ribosome_associated factors]]
[[Category: rna-binding]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 17:23:10 2007''