View source for 2g3k

[[Image:2g3k.gif|left|200px]]

 {{Structure
|PDB= 2g3k |SIZE=350|CAPTION= <scene name='initialview01'>2g3k</scene>, resolution 3.05&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
|ACTIVITY= 
|GENE= VPS28 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3k OCA], [http://www.ebi.ac.uk/pdbsum/2g3k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g3k RCSB]</span>
}}

'''Crystal structure of the C-terminal domain of Vps28'''


==Overview==
The endosomal sorting complex I required for transport (ESCRT-I) is composed of the three subunits Vps23/Tsg101, Vps28 and Vps37. ESCRT-I is recruited to cellular membranes during multivesicular endosome biogenesis and by enveloped viruses such as HIV-1 to mediate budding from the cell. Here, we describe the crystal structure of a conserved C-terminal domain from Sacharomyces cerevisiae Vps28 (Vps28-CTD) at 3.05 A resolution which folds independently into a four-helical bundle structure. Co-expression experiments of Vps28-CTD, Vps23 and Vps37 suggest that Vps28-CTD does not directly participate in ESCRT-I assembly and may thus act as an adaptor module for downstream interaction partners. We show through mutagenesis studies that Vps28-CTD employs its strictly conserved surface in the interaction with the ESCRT-III factor Vps20. Furthermore, we present evidence that Vps28-CTD is sufficient to rescue an equine infectious anaemia virus (EIAV) Gag late domain deletion. Vps28-CTD mutations abolishing Vps20 interaction in vitro also prevent the rescue of the EIAV Gag late domain mutant consistent with a potential direct Vps28-ESCRT-III Vps20 recruitment. Therefore, the physiological relevant EIAV Gag-Alix interaction can be functionally replaced by a Gag-Vps28-CTD fusion. Because both Alix and Vps28-CTD can directly recruit ESCRT-III proteins, ESCRT-III assembly coupled to Vps4 action may therefore constitute the minimal budding machinery for EIAV release.

==About this Structure==
2G3K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3K OCA]. 

==Reference==
The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment., Pineda-Molina E, Belrhali H, Piefer AJ, Akula I, Bates P, Weissenhorn W, Traffic. 2006 Aug;7(8):1007-16. Epub 2006 Jun 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16749904 16749904]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Akula, I.]]
[[Category: Bates, P.]]
[[Category: Belrhali, H.]]
[[Category: Piefer, A J.]]
[[Category: Pineda-Molina, E.]]
[[Category: Weissenhorn, W.]]
[[Category: 4 helix bundle]]

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