View source for 4ac5


==Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography==
<StructureSection load='4ac5' size='340' side='right' caption='[[4ac5]], [[Resolution|resolution]] 8.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ac5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AC5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AC5 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[7prc|7prc]], [[1dxr|1dxr]], [[2wjm|2wjm]], [[1prc|1prc]], [[2x5v|2x5v]], [[1vrn|1vrn]], [[3prc|3prc]], [[6prc|6prc]], [[1r2c|1r2c]], [[2wjn|2wjn]], [[2x5u|2x5u]], [[5prc|5prc]], [[2prc|2prc]], [[2jbl|2jbl]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ac5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ac5 OCA], [http://pdbe.org/4ac5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ac5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ac5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ac5 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/RCEM_RHOVI RCEM_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/CYCR_RHOVI CYCR_RHOVI]] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor. [[http://www.uniprot.org/uniprot/RCEL_RHOVI RCEL_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEH_RHOVI RCEH_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Lipidic phase membrane protein serial femtosecond crystallography.,Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U, Doak RB, Shoeman RL, Lomb L, Malmerberg E, Davidsson J, Nass K, Liang M, Andreasson J, Aquila A, Bajt S, Barthelmess M, Barty A, Bogan MJ, Bostedt C, Bozek JD, Caleman C, Coffee R, Coppola N, Ekeberg T, Epp SW, Erk B, Fleckenstein H, Foucar L, Graafsma H, Gumprecht L, Hajdu J, Hampton CY, Hartmann R, Hartmann A, Hauser G, Hirsemann H, Holl P, Hunter MS, Kassemeyer S, Kimmel N, Kirian RA, Maia FR, Marchesini S, Martin AV, Reich C, Rolles D, Rudek B, Rudenko A, Schlichting I, Schulz J, Seibert MM, Sierra RG, Soltau H, Starodub D, Stellato F, Stern S, Struder L, Timneanu N, Ullrich J, Wahlgren WY, Wang X, Weidenspointner G, Wunderer C, Fromme P, Chapman HN, Spence JC, Neutze R Nat Methods. 2012 Jan 29. doi: 10.1038/nmeth.1867. PMID:22286383<ref>PMID:22286383</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ac5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Blastochloris viridis]]
[[Category: Andreasson, J]]
[[Category: Aquila, A]]
[[Category: Arnlund, D]]
[[Category: Bajt, S]]
[[Category: Barthelmess, M]]
[[Category: Barty, A]]
[[Category: Bogan, M J]]
[[Category: Bostedt, C]]
[[Category: Bozek, J D]]
[[Category: Caleman, C]]
[[Category: Chapman, H N]]
[[Category: Coffee, R]]
[[Category: Coppola, N]]
[[Category: Davidsson, J]]
[[Category: DePonte, D P]]
[[Category: Doak, R B]]
[[Category: Ekeberg, T]]
[[Category: Epp, S W]]
[[Category: Erk, B]]
[[Category: Fleckenstein, H]]
[[Category: Foucar, L]]
[[Category: Fromme, P]]
[[Category: Graafsma, H]]
[[Category: Gumprecht, L]]
[[Category: Hajdu, J]]
[[Category: Hampton, C Y]]
[[Category: Hartmann, A]]
[[Category: Hartmann, R]]
[[Category: Hauser, G]]
[[Category: Hirsemann, H]]
[[Category: Holl, P]]
[[Category: Hunter, M S]]
[[Category: Johansson, L C]]
[[Category: Kassemeyer, S]]
[[Category: Katona, G]]
[[Category: Kimmel, N]]
[[Category: Kirian, R A]]
[[Category: Liang, M]]
[[Category: Lomb, L]]
[[Category: Maia, F R.N C]]
[[Category: Malmerberg, E]]
[[Category: Marchesini, S]]
[[Category: Martin, A V]]
[[Category: Nass, K]]
[[Category: Neutze, R]]
[[Category: Reich, C]]
[[Category: Rolles, D]]
[[Category: Rudek, B]]
[[Category: Rudenko, A]]
[[Category: Schlichting, I]]
[[Category: Schulz, J]]
[[Category: Seibert, M M]]
[[Category: Shoeman, R L]]
[[Category: Sierra, R]]
[[Category: Soltau, H]]
[[Category: Spence, J C.H]]
[[Category: Starodub, D]]
[[Category: Stellato, F]]
[[Category: Stern, S]]
[[Category: Struder, L]]
[[Category: Timneanu, N]]
[[Category: Ullrich, J]]
[[Category: Wahlgren, W Y]]
[[Category: Wang, X]]
[[Category: Weidenspointner, G]]
[[Category: Weierstall, U]]
[[Category: White, T A]]
[[Category: Wunderer, C]]
[[Category: Cell membrane]]
[[Category: Chromophore]]
[[Category: Electron transport]]
[[Category: Formylation]]
[[Category: Lipidic-sponge phase]]
[[Category: Lipoprotein]]
[[Category: Metal- binding]]
[[Category: Photosynthesis]]
[[Category: Reaction center]]
[[Category: Transmembrane]]