View source for 5y01


==Acid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) state==
<StructureSection load='5y01' size='340' side='right' caption='[[5y01]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5y01]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y01 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y01 OCA], [http://pdbe.org/5y01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y01 RCSB], [http://www.ebi.ac.uk/pdbsum/5y01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y01 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The fluorescent protein (FP) color palette has greatly contributed to the visualization of molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than their neutral pKa ( approximately 6), and this has hampered their application in acidic organelles (pH approximately 4.5-6.0). Currently, several cyan- and red-colored acid-tolerant FPs are available; however, there are few reports of acid-tolerant green FPs (GFPs) that are practically applicable to bioimaging. Here, we developed the acid-tolerant monomeric GFP "Gamillus" from the jellyfish Olindias formosa, with excellent brightness, maturation speed, and photostability. Results from X-ray crystallography and point mutagenesis suggest that across a broad pH range the acid tolerance is attributed to stabilization of deprotonation in the chromophore phenyl ring by forming a unique trans configuration. We demonstrate that Gamillus can serve as a molecular tag suitable for imaging in acidic organelles through autophagy-mediated molecular tracking to lysosomes.

Acid-Tolerant Monomeric GFP from Olindias formosa.,Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T Cell Chem Biol. 2017 Dec 23. pii: S2451-9456(17)30432-4. doi:, 10.1016/j.chembiol.2017.12.005. PMID:29290624<ref>PMID:29290624</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5y01" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Matsuda, T]]
[[Category: Nagai, T]]
[[Category: Nakashima, R]]
[[Category: Sakurai, K]]
[[Category: Shinoda, H]]
[[Category: Beta barrel]]
[[Category: Cis-chromophore]]
[[Category: Fluorescent protein]]
[[Category: Gfp-like protein]]