View source for 2c35

[[Image:2c35.gif|left|200px]]

 {{Structure
|PDB= 2c35 |SIZE=350|CAPTION= <scene name='initialview01'>2c35</scene>, resolution 2.7&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
|GENE= 
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c35 OCA], [http://www.ebi.ac.uk/pdbsum/2c35 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c35 RCSB]</span>
}}

'''SUBUNITS RPB4 AND RPB7 OF HUMAN RNA POLYMERASE II'''


==Overview==
The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II (RNAP(II)) form a heterodimer that protrudes from the 10-subunit core of the enzyme. We have obtained crystals of the human Rpb4/Rpb7 heterodimer and determined the structure to 2.7 A resolution. The presence of putative RNA-binding domains on the Rpb7 subunit and the position of the heterodimer close to the RNA exit groove in the 12 subunit yeast polymerase complex strongly suggests a role for the heterodimer in binding and stabilizing the nascent RNA transcript. We have complemented the structural analysis with biochemical studies directed at dissecting the RNA-binding properties of the human Rpb4/Rpb7 complex and that of the homologous E/F complex from Methanocaldococcus jannaschii. A number of conserved, solvent-exposed residues in both the human Rpb7 subunit and the archaeal E subunit have been modified by site-directed mutagenesis and the mutants tested for RNA binding by performing electrophoretic mobility shift assays. These studies have identified an elongated surface region on the corresponding face of both subunit E and Rpb7 that is involved in RNA binding. The area spans the nucleic acid binding face of the OB fold, including the B4-B5 loop, but also extends towards the N-terminal domain.

==About this Structure==
2C35 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C35 OCA]. 

==Reference==
Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II., Meka H, Werner F, Cordell SC, Onesti S, Brick P, Nucleic Acids Res. 2005 Nov 10;33(19):6435-44. Print 2005. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16282592 16282592]
[[Category: DNA-directed RNA polymerase]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Brick, P.]]
[[Category: Cordell, S C.]]
[[Category: Meka, H.]]
[[Category: Onesti, S.]]
[[Category: Werner, F.]]
[[Category: nucleotidyltransferase]]
[[Category: polymerase]]
[[Category: rna polymerase ii]]
[[Category: transcription]]

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