View source for 2bfh

[[Image:2bfh.jpg|left|200px]]

 {{Structure
|PDB= 2bfh |SIZE=350|CAPTION= <scene name='initialview01'>2bfh</scene>, resolution 2.5&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= 
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfh OCA], [http://www.ebi.ac.uk/pdbsum/2bfh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bfh RCSB]</span>
}}

'''CRYSTAL STRUCTURE OF BASIC FIBROBLAST GROWTH FACTOR AT 1.6 ANGSTROMS RESOLUTION'''


==Overview==
We have determined the crystal structures of two types of human basic fibroblast growth factor, the serine analogue and the wild-type, at 1.6 and 2.5 A resolution, respectively. Two good heavy atom derivatives were found and used for multiple isomorphous replacement phasing. The atomic coordinates were refined using the Hendrickson &amp; Konnert program for stereochemically restrained refinement against structure factors. The crystallographic R factors were reduced to 15.3% for the serine analogue structure and 16.0% for the wild-type structure. The serine analogue and wild-type structures have been found to be almost identical, the root-mean-square deviation between the corresponding C alpha atoms being 0.11 A. Their structures are composed of twelve beta-strands forming a barrel and three loops. Their molecules have an approximate threefold internal symmetry and are similar in architecture to that of interleukin-1 beta. A possible heparin-binding site, which comprises five basic residues, Lys119, Arg120, Lys125, Lys129, and Lys135, has been revealed by calculating the electrostatic potential energy.

==About this Structure==
2BFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFH OCA]. 

==Reference==
Crystal structure of basic fibroblast growth factor at 1.6 A resolution., Ago H, Kitagawa Y, Fujishima A, Matsuura Y, Katsube Y, J Biochem. 1991 Sep;110(3):360-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1769963 1769963]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Ago, H.]]
[[Category: Fujishima, A.]]
[[Category: Katsube, Y.]]
[[Category: Kitagawa, Y.]]
[[Category: Matsuura, Y.]]
[[Category: growth factor]]

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