View source for 1oxm

[[Image:1oxm.gif|left|200px]]<br />
<applet load="1oxm" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1oxm, resolution 2.30&Aring;" />
'''STRUCTURE OF CUTINASE'''<br />

==Overview==
Cutinase from Fusarium solani is a lipolytic enzyme that hydrolyses, triglycerides efficiently. All the inhibited forms of lipolytic enzymes, described so far are based on the use of small organophosphate and, organophosphonate inhibitors, which bear little resemblance to a natural, triglyceride substrate. In this article we describe the crystal structure, of cutinase covalently inhibited by, (R)-1,2-dibutyl-carbamoylglycero-3-O-p-nitrophenylbutyl-phos phonate, a, triglyceride analogue mimicking the first tetrahedral intermediate along, the reaction pathway. The structure, which has been solved at 2.3 A, reveals that in both the protein molecules of the asymmetric unit the, inhibitor is almost completely embedded in the active site crevice. The, overall shape of the inhibitor is that of a fork: the two, dibutyl-carbamoyl chains point towards the surface of the protein, whereas, the butyl chain bound to the phosphorous atom is roughly perpendicular to, the sn-1 and sn-2 chains. The sn-3 chain is accommodated in a rather small, pocket at the bottom of the active site crevice, thus providing a, structural explanation for the preference of cutinase for short acyl chain, substrates.

==About this Structure==
1OXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi] with TC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: TC4. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OXM OCA]. 

==Reference==
Crystal structure of cutinase covalently inhibited by a triglyceride analogue., Longhi S, Mannesse M, Verheij HM, De Haas GH, Egmond M, Knoops-Mouthuy E, Cambillau C, Protein Sci. 1997 Feb;6(2):275-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9041628 9041628]
[[Category: Fusarium solani subsp. pisi]]
[[Category: Single protein]]
[[Category: Cambillau, C.]]
[[Category: Longhi, S.]]
[[Category: TC4]]
[[Category: glycoprotein]]
[[Category: hydrolase]]
[[Category: serine esterase]]

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