View source for 4yi9


==Crystal structure of non-myristoylated E153A recoverin at 1.35 A resolution with a sodium ion bound to EF-hand 2 and calcium ion bound to EF-hand 3==
<StructureSection load='4yi9' size='340' side='right' caption='[[4yi9]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4yi9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YI9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YI9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mlw|4mlw]], [[4yi8|4yi8]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RCVRN, RCV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yi9 OCA], [http://pdbe.org/4yi9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yi9 RCSB], [http://www.ebi.ac.uk/pdbsum/4yi9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yi9 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/RECO_BOVIN RECO_BOVIN]] Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.<ref>PMID:8097896</ref> <ref>PMID:8392055</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recoverin (Rv), a small Ca2+-binding protein that inhibits rhodopsin kinase (RK), has four EF hands, two of which are functional (EF2 and EF3). Activation requires Ca2+ in both EF hands, but crystal structures have never been observed with Ca2+ ions in both sites; all previous structures have Ca2+ bound only to EF3. We suspected that this was due to an intermolecular crystal contact between T80 and a surface glutamate (E153) that precluded coordination of a Ca2+ ion in EF2. We constructed the E153A mutant, determined its X-ray crystal structure to 1.2 A resolution, and show that 2 Ca2+ ions are bound, one in EF3 and one in EF2. Additionally, several other residues are shown to adopt conformations in the 2Ca2+-structure not seen previously and not seen in a second structure of the E153A mutant containing Na+ instead of Ca2+ in the EF2 site. The side-chain rearrangements in these residues form a 28 A long allosteric cascade along the surface of the protein connecting the Ca2+-binding site of EF2 with the active-site pocket responsible for binding RK.

Crystal Structure of Recoverin with Calcium Ions Bound to Both Functional EF-Hands.,Kumar RP, Ranaghan MJ, Ganjei AY, Oprian DD Biochemistry. 2015 Nov 19. PMID:26584024<ref>PMID:26584024</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4yi9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bovin]]
[[Category: Kumar, R Prem]]
[[Category: Oprian, D D]]
[[Category: Ranaghan, M J]]
[[Category: Calcium binding protein]]
[[Category: Ef hand]]
[[Category: Ncs family]]