View source for 5prm


==PanDDA analysis group deposition -- Crystal Structure of BRD1 after initial refinement with no ligand modelled (structure 94)==
<StructureSection load='5prm' size='340' side='right' caption='[[5prm]], [[Resolution|resolution]] 3.58&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5prm]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PRM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5PRM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5prm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5prm OCA], [http://pdbe.org/5prm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5prm RCSB], [http://www.ebi.ac.uk/pdbsum/5prm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5prm ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/BRD1_HUMAN BRD1_HUMAN]] Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.<ref>PMID:16387653</ref> <ref>PMID:21880731</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In macromolecular crystallography, the rigorous detection of changed states (for example, ligand binding) is difficult unless signal is strong. Ambiguous ('weak' or 'noisy') density is experimentally common, since molecular states are generally only fractionally present in the crystal. Existing methodologies focus on generating maximally accurate maps whereby minor states become discernible; in practice, such map interpretation is disappointingly subjective, time-consuming and methodologically unsound. Here we report the PanDDA method, which automatically reveals clear electron density for the changed state-even from inaccurate maps-by subtracting a proportion of the confounding 'ground state'; changed states are objectively identified from statistical analysis of density distributions. The method is completely general, implying new best practice for all changed-state studies, including the routine collection of multiple ground-state crystals. More generally, these results demonstrate: the incompleteness of atomic models; that single data sets contain insufficient information to model them fully; and that accuracy requires further map-deconvolution approaches.

A multi-crystal method for extracting obscured crystallographic states from conventionally uninterpretable electron density.,Pearce NM, Krojer T, Bradley AR, Collins P, Nowak RP, Talon R, Marsden BD, Kelm S, Shi J, Deane CM, von Delft F Nat Commun. 2017 Apr 24;8:15123. doi: 10.1038/ncomms15123. PMID:28436492<ref>PMID:28436492</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5prm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arrowsmith, C H]]
[[Category: Bountra, C]]
[[Category: Bradley, A R]]
[[Category: Brandao-Neto, J]]
[[Category: Brennan, P E]]
[[Category: Collins, P]]
[[Category: Cox, O]]
[[Category: Delft, F von]]
[[Category: Dias, A]]
[[Category: Douangamath, A]]
[[Category: Edwards, A]]
[[Category: Fairhead, M]]
[[Category: Krojer, T]]
[[Category: MacLean, E]]
[[Category: Ng, J]]
[[Category: Pearce, N M]]
[[Category: Renjie, Z]]
[[Category: Sethi, R]]
[[Category: Talon, R]]
[[Category: Wright, N]]
[[Category: Bromodomain]]
[[Category: Epigenetic]]
[[Category: Gene regulation]]
[[Category: Pandda]]
[[Category: Sgc - diamond i04-1 fragment screening]]
[[Category: Xchemexplorer]]