View source for 1wr5

[[Image:1wr5.gif|left|200px]]

 {{Structure
|PDB= 1wr5 |SIZE=350|CAPTION= <scene name='initialview01'>1wr5</scene>, resolution 1.40&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
|ACTIVITY= 
|GENE= 
|DOMAIN=
|RELATEDENTRY=[[2cdv|2CDV]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wr5 OCA], [http://www.ebi.ac.uk/pdbsum/1wr5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wr5 RCSB]</span>
}}

'''Three dimensional Structure of the E41K mutant of Tetraheme Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F'''


==Overview==
Tyrosine 43 is positioned parallel to the fifth heme axial ligand, His34, of heme 1 in the tetraheme cytochrome c(3). The replacement of tyrosine with leucine increased the redox potential of heme 1 by 44 and 35 mV at the first and last reduction steps, respectively; its effects on the other hemes are small. In contrast, the Y43F mutation hardly changed the potentials. It shows that the aromatic ring at this position contributes to lowering the redox potential of heme 1 locally, although this cannot be the major contribution to the extremely low redox potentials of cytochrome c(3). Furthermore, temperature-dependent line-width broadening in partially reduced samples established that the aromatic ring at position 43 participates in the control of the kinetics of intramolecular electron transfer. The rate of reduction of Y43L cytochrome c(3) by 5-deazariboflavin semiquinone under partially reduced conditions was significantly different from that of the wild type in the last stage of the reduction, supporting the involvement of Tyr43 in regulation of reduction kinetics. The mutation of Y43L, however, did not induce a significant change in the crystal structure.

==About this Structure==
1WR5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WR5 OCA]. 

==Reference==
Role of the aromatic ring of Tyr43 in tetraheme cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F., Ozawa K, Takayama Y, Yasukawa F, Ohmura T, Cusanovich MA, Tomimoto Y, Ogata H, Higuchi Y, Akutsu H, Biophys J. 2003 Nov;85(5):3367-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14581238 14581238]
[[Category: Desulfovibrio vulgaris]]
[[Category: Single protein]]
[[Category: Higuchi, Y.]]
[[Category: Ogata, H.]]
[[Category: Tomimoto, Y.]]
[[Category: e41k mutant]]
[[Category: high resolution x-ray structure]]
[[Category: tetraheme cytochrome c3]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:40:00 2008''