View source for 5y2x


==Crystal structure of apo-HaloTag (M175C)==
<StructureSection load='5y2x' size='340' side='right' caption='[[5y2x]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5y2x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y2X FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y2x OCA], [http://pdbe.org/5y2x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y2x RCSB], [http://www.ebi.ac.uk/pdbsum/5y2x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y2x ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Alkyl halides are potentially mutagenic carcinogens. However, no efficient fluorescent sensor for alkyl halide detection in human-derived samples has been developed to date. Herein, we report a new protein-based fluorescent sensor for alkyl halides. Analysis of the HaloTag holo-crystal structure with its covalently attached ligand revealed an unexpected cavity, allowing for the design of a new fluorogenic ligand. This ligand showed the highest fluorescence response (300-fold) and fastest binding kinetics (t1/2 &lt; 150 s) to a HaloTag mutant (M175P) protein. This protein-based sensor system was effectively used to detect alkyl halides in human serum and monitor real-time protein alkylation.

Structure-guided synthesis of a protein-based fluorescent sensor for alkyl halides.,Kang MG, Lee H, Kim BH, Dunbayev Y, Seo JK, Lee C, Rhee HW Chem Commun (Camb). 2017 Aug 15;53(66):9226-9229. doi: 10.1039/c7cc03714g. PMID:28766590<ref>PMID:28766590</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5y2x" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Haloalkane dehalogenase]]
[[Category: Kang, M]]
[[Category: Lee, C]]
[[Category: Lee, H]]
[[Category: Rhee, H]]
[[Category: Halotag]]
[[Category: Hydrolase]]