View source for 1uue

[[Image:1uue.jpg|left|200px]]

 {{Structure
|PDB= 1uue |SIZE=350|CAPTION= <scene name='initialview01'>1uue</scene>, resolution 2.60&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= 
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uue OCA], [http://www.ebi.ac.uk/pdbsum/1uue PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uue RCSB]</span>
}}

'''A-SPECTRIN SH3 DOMAIN (V44T, D48G MUTANT)'''


==Overview==
An effort to combine theoretical analyses and protein engineering methods has been made to probe the folding mechanism of SH3 by using Energy Landscape Theory and a phi-value analysis. Particular emphasis was given to core residues and the effect of desolvation during the folding event by replacing the core valines with isosteric threonines. These mutations have the advantage of keeping the core structurally invariant while affecting core stability relative to the unfolded state. Although the valines that form the core appear spatially invariant, the folding kinetics of their threonine mutants varies, indicating their different extent of solvation in the transition-state ensemble. Theoretical studies predicted the distribution of folding kinetics of threonine mutants without previous knowledge of the measured rates. This initial success encourages further investigations of the molecular details behind these macroscopic phenomena and of the role of solvation in the folding mechanism.

==About this Structure==
1UUE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UUE OCA]. 

==Reference==
Solvation in protein folding analysis: combination of theoretical and experimental approaches., Fernandez-Escamilla AM, Cheung MS, Vega MC, Wilmanns M, Onuchic JN, Serrano L, Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2834-9. Epub 2004 Feb 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14978284 14978284]
[[Category: Gallus gallus]]
[[Category: Single protein]]
[[Category: Fernandez, A.]]
[[Category: Serrano, L.]]
[[Category: Vega, M C.]]
[[Category: Wilmanns, M.]]
[[Category: actin-binding]]
[[Category: calcium-binding]]
[[Category: calmodulin-binding]]
[[Category: cytoskeleton]]
[[Category: membrane]]
[[Category: sh3]]
[[Category: sh3-domain]]
[[Category: spectrin]]

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