View source for 4xtl


==Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3==
<StructureSection load='4xtl' size='340' side='right' caption='[[4xtl]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xtl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XTL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XTL FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xtl OCA], [http://pdbe.org/4xtl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xtl RCSB], [http://www.ebi.ac.uk/pdbsum/4xtl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xtl ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus rhodopsin 2 (KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 A and 2.2 and 2.8 A, respectively. The structures reveal the ion-translocation pathway and show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal alpha-helix and that the ion-uptake cavity is unexpectedly large and open to the surface. Obstruction of the cavity with the mutation G263F imparts KR2 with the ability to pump potassium. These results pave the way for the understanding and rational design of cation pumps with new specific properties valuable for optogenetics.

Crystal structure of a light-driven sodium pump.,Gushchin I, Shevchenko V, Polovinkin V, Kovalev K, Alekseev A, Round E, Borshchevskiy V, Balandin T, Popov A, Gensch T, Fahlke C, Bamann C, Willbold D, Buldt G, Bamberg E, Gordeliy V Nat Struct Mol Biol. 2015 Apr 6. doi: 10.1038/nsmb.3002. PMID:25849142<ref>PMID:25849142</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xtl" style="background-color:#fffaf0;"></div>

==See Also==
*[[Rhodopsin|Rhodopsin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gordeliy, V]]
[[Category: Gushchin, I]]
[[Category: Polovinkin, V]]
[[Category: Shevchenko, V]]
[[Category: Ion pump]]
[[Category: Membrane protein]]