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==Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with AdoMet and with DNA Containing Proximal Pap Regulon Sequence==
<StructureSection load='4rtp' size='340' side='right' caption='[[4rtp]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rtp]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RTP FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2g1p|2g1p]], [[2ore|2ore]], [[4rtj|4rtj]], [[4rtk|4rtk]], [[4rtl|4rtl]], [[4rtm|4rtm]], [[4rtn|4rtn]], [[4rto|4rto]], [[4rtq|4rtq]], [[4rtr|4rtr]], [[4rts|4rts]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rtp OCA], [http://pdbe.org/4rtp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rtp RCSB], [http://www.ebi.ac.uk/pdbsum/4rtp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rtp ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DNA adenine methyltransferase (Dam) is widespread and conserved among the gamma-proteobacteria. Methylation of the Ade in GATC sequences regulates diverse bacterial cell functions, including gene expression, mismatch repair and chromosome replication. Dam also controls virulence in many pathogenic Gram-negative bacteria. An unexplained and perplexing observation about Escherichia coli Dam (EcoDam) is that there is no obvious relationship between the genes that are transcriptionally responsive to Dam and the promoter-proximal presence of GATC sequences. Here, we demonstrate that EcoDam interacts with a 5-base pair non-cognate sequence distinct from GATC. The crystal structure of a non-cognate complex allowed us to identify a DNA binding element, GTYTA/TARAC (where Y = C/T and R = A/G). This element immediately flanks GATC sites in some Dam-regulated promoters, including the Pap operon which specifies pyelonephritis-associated pili. In addition, Dam interacts with near-cognate GATC sequences (i.e. 3/4-site ATC and GAT). Taken together, these results imply that Dam, in addition to being responsible for GATC methylation, could also function as a methylation-independent transcriptional repressor.

Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.,Horton JR, Zhang X, Blumenthal RM, Cheng X Nucleic Acids Res. 2015 Apr 6. pii: gkv251. PMID:25845600<ref>PMID:25845600</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4rtp" style="background-color:#fffaf0;"></div>

==See Also==
*[[DNA adenine methylase|DNA adenine methylase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cheng, X]]
[[Category: Horton, J R]]
[[Category: Bacterial virulence]]
[[Category: Base flipping]]
[[Category: Dam methylation]]
[[Category: Gatc recognition]]
[[Category: Methylation-independent transcriptional repressor]]
[[Category: Transferase-dna complex]]