View source for 3tk1


==Crystal structure of a MeaB and Rv1496 ortholog from Mycobacterium thermoresistible bound to GDP==
<StructureSection load='3tk1' size='340' side='right' caption='[[3tk1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3tk1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_thermoresistibile_atcc_19527 Mycobacterium thermoresistibile atcc 19527]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TK1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3md0|3md0]], [[3p32|3p32]], [[3nxs|3nxs]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KEK_00260 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1078020 Mycobacterium thermoresistibile ATCC 19527])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk1 OCA], [http://pdbe.org/3tk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tk1 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk1 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The methylmalonyl Co-A mutase-associated GTPase MeaB from Methylobacterium extorquens is involved in glyoxylate regulation and required for growth. In humans, mutations in the homolog methylmalonic aciduria associated protein (MMAA) cause methylmalonic aciduria, which is often fatal. The central role of MeaB from bacteria to humans suggests that MeaB is also important in other, pathogenic bacteria such as Mycobacterium tuberculosis. However, the identity of the mycobacterial MeaB homolog is presently unclear. Here, we identify the M. tuberculosis protein Rv1496 and its homologs in M. smegmatis and M. thermoresistibile as MeaB. The crystal structures of all three homologs are highly similar to MeaB and MMAA structures and reveal a characteristic three-domain homodimer with GDP bound in the G domain active site. A structure of Rv1496 obtained from a crystal grown in the presence of GTP exhibited electron density for GDP, suggesting GTPase activity. These structures identify the mycobacterial MeaB and provide a structural framework for therapeutic targeting of M. tuberculosis MeaB.

Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.,Edwards TE, Baugh L, Bullen J, Baydo RO, Witte P, Thompkins K, Phan IQ, Abendroth J, Clifton MC, Sankaran B, Van Voorhis WC, Myler PJ, Staker BL, Grundner C, Lorimer DD J Struct Funct Genomics. 2015 Jun;16(2):91-9. doi: 10.1007/s10969-015-9197-2., Epub 2015 Apr 2. PMID:25832174<ref>PMID:25832174</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Mycobacterium thermoresistibile atcc 19527]]
[[Category: Structural genomic]]
[[Category: G-protein]]
[[Category: Gdp]]
[[Category: Hydrolase]]
[[Category: Meab]]
[[Category: Methylmalonic aciduria]]
[[Category: Mmaa]]
[[Category: Ras-like gtpase]]
[[Category: Rv1496]]
[[Category: Ssgcid]]
[[Category: Thermophile]]