View source for 1rxv

[[Image:1rxv.gif|left|200px]]

 {{Structure
|PDB= 1rxv |SIZE=350|CAPTION= <scene name='initialview01'>1rxv</scene>, resolution 2.5&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
|ACTIVITY= 
|GENE= FEN, AF0264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])
|DOMAIN=
|RELATEDENTRY=[[1rwz|1RWZ]], [[1rxq|1RXQ]], [[1rxw|1RXW]], [[1rxz|1RXZ]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rxv OCA], [http://www.ebi.ac.uk/pdbsum/1rxv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rxv RCSB]</span>
}}

'''Crystal Structure of A. Fulgidus FEN-1 bound to DNA'''


==Overview==
Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.

==About this Structure==
1RXV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXV OCA]. 

==Reference==
Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14718165 14718165]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Chapados, B R.]]
[[Category: Han, S.]]
[[Category: Hosfield, D J.]]
[[Category: Qiu, J.]]
[[Category: Shen, B.]]
[[Category: Tainer, J A.]]
[[Category: Yelent, B.]]
[[Category: 3' flap binding site]]
[[Category: dna repair]]
[[Category: dna replication]]
[[Category: helical clamp]]
[[Category: helix-3 turn-helix]]
[[Category: protein-dna complex]]

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