View source for 4pz4


==High-resolution crystal structure of the human CD44 hyaluronan binding domain in new space group==
<StructureSection load='4pz4' size='340' side='right' caption='[[4pz4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pz4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PZ4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1uuh|1uuh]], [[4pz3|4pz3]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pz4 OCA], [http://pdbe.org/4pz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4pz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pz4 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CD44_HUMAN CD44_HUMAN]] Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. 
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== Publication Abstract from PubMed ==
Two new crystal structures of the extracellular hyaluronan-binding domain of human CD44 are described at high resolution. A hexagonal crystal form at 1.60 A resolution and a monoclinic form at 1.08 A resolution both have two molecules in the asymmetric unit arranged about a similar noncrystallographic twofold axis of symmetry. These structures are compared with those previously reported at 2.20 A resolution to show that the fold is quite resistant to structural deformation in different crystal environments. Unexpectedly, a short peptide is found in the monoclinic crystals at a site remote from the known hyaluronan-binding groove. The peptide with a valine at the carboxy-terminus must have co-purified from the bacterial expression host and binds on the opposite side of the domain from the known hyaluronan-binding groove. This opportunistic binding may identify a site of interaction used as CD44 assembles with other proteins to accomplish effective signaling regarding changes to the extracellular environment.

High-resolution crystal structures of alternate forms of the human CD44 hyaluronan-binding domain reveal a site for protein interaction.,Liu LK, Finzel B Acta Crystallogr F Struct Biol Commun. 2014 Sep 1;70(Pt 9):1155-61. doi:, 10.1107/S2053230X14015532. Epub 2014 Aug 29. PMID:25195884<ref>PMID:25195884</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[CD44|CD44]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Finzel, B]]
[[Category: Liu, L K]]
[[Category: Binding protein]]
[[Category: Cell adhesion]]
[[Category: Ectodomain]]
[[Category: Hyaluronan]]
[[Category: Link module]]