View source for 4ozq


==Crystal structure of the mouse Kif14 motor domain==
<StructureSection load='4ozq' size='340' side='right' caption='[[4ozq]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ozq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OZQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ozq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ozq OCA], [http://pdbe.org/4ozq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ozq RCSB], [http://www.ebi.ac.uk/pdbsum/4ozq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ozq ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mitotic kinesin motor protein KIF14 is essential for cytokinesis during cell division, and has been implicated in cerebral development and a variety of human cancers. Here we show that the mouse KIF14 motor domain binds tightly to microtubules and does not display typical nucleotide-dependent changes in this affinity. It also has robust ATPase activity but very slow motility. A crystal structure of the ADP-bound form of the KIF14 motor domain reveals a dramatically opened ATP binding pocket, as if ready to exchange its bound ADP for Mg.ATP. In this state, the central beta-sheet is twisted ~10 degrees beyond the maximal amount observed in other kinesins. This configuration has only been seen in the nucleotide-free states of myosins - known as the 'rigor-like' state. Fitting of this atomic model to electron density maps from cryo-electron microscopy indicates a distinct binding configuration of the motor domain to microtubules. We postulate that these properties of KIF14 are well-suited for stabilizing midbody microtubules during cytokinesis.

KIF14 binds tightly to microtubules and adopts a rigor-like conformation.,Arora K, Talje L, Asenjo AB, Andersen P, Atchia K, Joshi M, Sosa H, Allingham JS, Kwok BH J Mol Biol. 2014 Jun 17. pii: S0022-2836(14)00282-4. doi:, 10.1016/j.jmb.2014.05.030. PMID:24949858<ref>PMID:24949858</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ozq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Allingham, J S]]
[[Category: Andersen, P]]
[[Category: Arora, K]]
[[Category: Asenjo, A B]]
[[Category: Atchia, K]]
[[Category: Joshi, M]]
[[Category: Kwok, B H]]
[[Category: Sosa, H]]
[[Category: Talje, L]]
[[Category: Atpase]]
[[Category: Kinesin]]
[[Category: Motor protein]]