View source for 4lg5


==ABA-mimicking ligand QUINABACTIN in complex with ABA receptor PYL2 and PP2C HAB1==
<StructureSection load='4lg5' size='340' side='right' caption='[[4lg5]], [[Resolution|resolution]] 2.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4lg5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LG5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LG5 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A1O:QUINABACTIN'>A1O</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lga|4lga]], [[4lgb|4lgb]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PYL2, RCAR14, At2g26040, T19L18.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), HAB1, P2C-HA, At1g72770, F28P22.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lg5 OCA], [http://pdbe.org/4lg5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lg5 RCSB], [http://www.ebi.ac.uk/pdbsum/4lg5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lg5 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PYL2_ARATH PYL2_ARATH]] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA.<ref>PMID:19898420</ref> <ref>PMID:19893533</ref>  [[http://www.uniprot.org/uniprot/P2C16_ARATH P2C16_ARATH]] Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Confers enhanced sensitivity to drought.<ref>PMID:14731256</ref> <ref>PMID:16876791</ref> <ref>PMID:16798945</ref> <ref>PMID:19033529</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Abscisic acid (ABA) is the most important hormone for plants to resist drought and other abiotic stresses. ABA binds directly to the PYR/PYL family of ABA receptors, resulting in inhibition of type 2C phosphatases (PP2C) and activation of downstream ABA signaling. It is envisioned that intervention of ABA signaling by small molecules could help plants to overcome abiotic stresses such as drought, cold and soil salinity. However, chemical instability and rapid catabolism by plant enzymes limit the practical application of ABA itself. Here we report the identification of a small molecule ABA mimic (AM1) that acts as a potent activator of multiple members of the family of ABA receptors. In Arabidopsis, AM1 activates a gene network that is highly similar to that induced by ABA. Treatments with AM1 inhibit seed germination, prevent leaf water loss, and promote drought resistance. We solved the crystal structure of AM1 in complex with the PYL2 ABA receptor and the HAB1 PP2C, which revealed that AM1 mediates a gate-latch-lock interacting network, a structural feature that is conserved in the ABA-bound receptor/PP2C complex. Together, these results demonstrate that a single small molecule ABA mimic can activate multiple ABA receptors and protect plants from water loss and drought stress. Moreover, the AM1 complex crystal structure provides a structural basis for designing the next generation of ABA-mimicking small molecules.

An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants.,Cao M, Liu X, Zhang Y, Xue X, Zhou XE, Melcher K, Gao P, Wang F, Zeng L, Zhao Y, Zhao Y, Deng P, Zhong D, Zhu JK, Xu HE, Xu Y Cell Res. 2013 Aug;23(8):1043-54. doi: 10.1038/cr.2013.95. Epub 2013 Jul 9. PMID:23835477<ref>PMID:23835477</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4lg5" style="background-color:#fffaf0;"></div>

==See Also==
*[[PYR/PYL/RCAR family of ABA receptors|PYR/PYL/RCAR family of ABA receptors]]
*[[Protein phosphatase|Protein phosphatase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arath]]
[[Category: Phosphoprotein phosphatase]]
[[Category: Deng, P]]
[[Category: Gao, M]]
[[Category: Gao, P]]
[[Category: Liu, X]]
[[Category: Melcher, K]]
[[Category: Wang, F]]
[[Category: Xu, H E]]
[[Category: Xu, Y]]
[[Category: Xue, X]]
[[Category: Zeng, L]]
[[Category: Zhang, Y]]
[[Category: Zhao, Y]]
[[Category: Zhong, D]]
[[Category: Zhou, X E]]
[[Category: Zhu, J K]]
[[Category: Abscisic acid mimicking compound]]
[[Category: Hydrolase-receptor-inhibitor complex]]
[[Category: Pp2c]]
[[Category: Pyr/pyl/rcar]]