View source for 4j2r


==Middle domain of influenza A virus RNA-dependent polymerase PB2==
<StructureSection load='4j2r' size='340' side='right' caption='[[4j2r]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4j2r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/I34a1 I34a1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J2R FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3cw4|3cw4]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=211044 I34A1])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2r OCA], [http://pdbe.org/4j2r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j2r RCSB], [http://www.ebi.ac.uk/pdbsum/4j2r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2r ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PB2_I34A1 PB2_I34A1]] Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication (By similarity). 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In the last hundred years, four influenza pandemics have been experienced, beginning with that in Spain in 1918. Influenza A virus causes severe pneumonia and its RNA polymerase is an important target for drug design. The influenza A (H1N1) virus has eight ribonucleoprotein complexes, which are composed of viral RNA, RNA polymerases and nucleoproteins. PB2 forms part of the RNA polymerase complex and plays an important role in binding to the cap structure of host mRNA. The middle domain of PB2 includes a cap-binding site. The structure of PB2 from H1N1 complexed with m(7)GTP has not been reported. Plate-like crystals of the middle domain of PB2 from H1N1 were obtained, but the quality of these crystals was not good. An attempt was made to crystallize the middle domain of PB2 complexed with m(7)GTP using a soaking method; however, electron density for m(7)GTP was not observed on preliminary X-ray diffraction analysis. This protein has hydrophobic residues on its surface and is stable in the presence of high salt concentrations. To improve the solubility, a surface double mutant (P453H and I471T) was prepared. These mutations change the surface electrostatic potential drastically. The protein was successfully prepared at a lower salt concentration and good cube-shaped crystals were obtained using this protein. Here, the crystallization and preliminary X-ray diffraction analysis of this mutant of the middle domain of PB2 are reported.

Crystallization and preliminary X-ray diffraction studies of a surface mutant of the middle domain of PB2 from human influenza A (H1N1) virus.,Tsurumura T, Qiu H, Yoshida T, Tsumori Y, Tsuge H Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):72-5. doi:, 10.1107/S2053230X13032603. Epub 2013 Dec 24. PMID:24419622<ref>PMID:24419622</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4j2r" style="background-color:#fffaf0;"></div>

==See Also==
*[[RNA polymerase|RNA polymerase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: I34a1]]
[[Category: Hatakeyama, D]]
[[Category: Kuzuhara, T]]
[[Category: Qiu, H]]
[[Category: Tsuge, H]]
[[Category: Tsumori, Y]]
[[Category: Tsurumura, T]]
[[Category: Cap-binding domain]]
[[Category: Cap-snatching]]
[[Category: Viral protein]]