View source for 3plu


==Structure of Hub-1 protein in complex with Snu66 peptide (HINDI)==
<StructureSection load='3plu' size='340' side='right' caption='[[3plu]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3plu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PLU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PLU FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3plv|3plv]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HUB1, YNR032C-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3plu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3plu OCA], [http://pdbe.org/3plu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3plu RCSB], [http://www.ebi.ac.uk/pdbsum/3plu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3plu ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/HUB1_YEAST HUB1_YEAST]] Forms conjugate with SPH1 and HBT1. Involved in morphogenesis. [[http://www.uniprot.org/uniprot/SNU66_YEAST SNU66_YEAST]] Component of the U4/U6.U5 tri-snRNP particle, one of the building blocks of the spliceosome. Required for pre-mRNA splicing.<ref>PMID:11290703</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Alternative splicing of pre-messenger RNAs diversifies gene products in eukaryotes and is guided by factors that enable spliceosomes to recognize particular splice sites. Here we report that alternative splicing of Saccharomyces cerevisiae SRC1 pre-mRNA is promoted by the conserved ubiquitin-like protein Hub1. Structural and biochemical data show that Hub1 binds non-covalently to a conserved element termed HIND, which is present in the spliceosomal protein Snu66 in yeast and mammals, and Prp38 in plants. Hub1 binding mildly alters spliceosomal protein interactions and barely affects general splicing in S. cerevisiae. However, spliceosomes that lack Hub1, or are defective in Hub1-HIND interaction, cannot use certain non-canonical 5' splice sites and are defective in alternative SRC1 splicing. Hub1 confers alternative splicing not only when bound to HIND, but also when experimentally fused to Snu66, Prp38, or even the core splicing factor Prp8. Our study indicates a novel mechanism for splice site utilization that is guided by non-covalent modification of the spliceosome by an unconventional ubiquitin-like modifier.

Role of the ubiquitin-like protein Hub1 in splice-site usage and alternative splicing.,Mishra SK, Ammon T, Popowicz GM, Krajewski M, Nagel RJ, Ares M, Holak TA, Jentsch S Nature. 2011 May 25. PMID:21614000<ref>PMID:21614000</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3plu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nucleoprotein|Nucleoprotein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Ammon, T]]
[[Category: Ares, M]]
[[Category: Holak, T A]]
[[Category: Jentsch, S]]
[[Category: Krajewski, M]]
[[Category: Mishra, S K]]
[[Category: Nagel, R J]]
[[Category: Popowicz, G M]]
[[Category: Hub-1]]
[[Category: Peptide binding protein]]
[[Category: Snu66]]
[[Category: Ubiquitin-like]]