View source for 1lt9

[[Image:1lt9.jpg|left|200px]]

 {{Structure
|PDB= 1lt9 |SIZE=350|CAPTION= <scene name='initialview01'>1lt9</scene>, resolution 2.80&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
|ACTIVITY= 
|GENE= 
|DOMAIN=
|RELATEDENTRY=[[1fza|1FZA]], [[1fzb|1FZB]], [[1fzc|1FZC]], [[1fze|1FZE]], [[1fzf|1FZF]], [[1fzg|1FZG]], [[1ltj|1LTJ]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lt9 OCA], [http://www.ebi.ac.uk/pdbsum/1lt9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lt9 RCSB]</span>
}}

'''Crystal Structure of Recombinant Human Fibrinogen Fragment D'''


==Overview==
We report two crystal structures, each at a resolution of 2.8 A, of recombinant human fibrinogen fragment D (rfD) in the absence and presence of peptide ligands. The bound ligands, Gly-Pro-Arg-Pro-amide and Gly-His-Arg-Pro-amide, mimic the interactions of the thrombin exposed polymerization sites, "A" and "B", respectively. This report is the first to describe the structure of fragment D in the presence of both peptide ligands. The structures reveal that recombinant fibrinogen is nearly identical to the plasma protein but with minor changes, like the addition of a proximal fucose to the carbohydrate linked to residue betaGln364, and slightly different relative positions of the beta- and gamma-modules. Of major interest in our structures is that a previously identified calcium site in plasma fibrinogen is absent when Gly-His-Arg-Pro-amide is bound. The peptide-dependent loss of this calcium site may have significant biological implications that are further discussed. These structures provide a foundation for the detailed structural analysis of variant recombinant fibrinogens that were used to identify critical functional residues within fragment D.

==About this Structure==
1LT9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LT9 OCA]. 

==Reference==
2.8 A crystal structures of recombinant fibrinogen fragment D with and without two peptide ligands: GHRP binding to the "b" site disrupts its nearby calcium-binding site., Kostelansky MS, Betts L, Gorkun OV, Lord ST, Biochemistry. 2002 Oct 8;41(40):12124-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12356313 12356313]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Betts, L.]]
[[Category: Gorkun, O V.]]
[[Category: Kostelansky, M S.]]
[[Category: Lord, S T.]]
[[Category: blood coagulation]]
[[Category: fibrinogen]]
[[Category: fibrinogen fragment d]]
[[Category: recombinant fibrinogen]]
[[Category: recombinant fibrinogen fragment d]]

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