View source for 4nj2


==GCN4-p1 triple Val9, 23,30 to Ile mutant==
<StructureSection load='4nj2' size='340' side='right' caption='[[4nj2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4nj2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJ2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zta|2zta]], [[4dmd|4dmd]], [[4niz|4niz]], [[4nj0|4nj0]], [[4nj1|4nj1]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nj2 OCA], [http://pdbe.org/4nj2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4nj2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nj2 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'. 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nature uses proteins and nucleic acids to form a wide array of functional architectures, and scientists have found inspiration from these structures in the rational design of synthetic biomaterials. We have recently shown that a modular subunit consisting of two alpha-helical coiled coil peptides attached at their midpoints by an organic linking group can spontaneously self-assemble in aqueous solution to form a soluble supramolecular polymer. Here we explore the use of coiled-coil association affinity, readily tuned by amino acid sequence, as a means to predictably alter properties of these supramolecular assemblies. A series of dimeric coiled-coil peptide sequences with identical quaternary folded structures but systematically altered folded stability were designed and biophysically characterized. The sequences were cross-linked to generate a series of branched, self-assembling biomacromolecular subunits. A clear relationship is observed between coiled-coil association affinity and apparent hydrodynamic diameter of the supramolecular polymers formed by these subunits. Our results provide a family of soluble supramolecular polymers of tunable size and well-characterized coiled-coil sequences that add to the library of building blocks available for use in the rational design of protein-based supramolecular biomaterials.

Tuning assembly size in Peptide-based supramolecular polymers by modulation of subunit association affinity.,Oshaben KM, Horne WS Biomacromolecules. 2014 Apr 14;15(4):1436-42. doi: 10.1021/bm5000423. Epub 2014, Mar 17. PMID:24598042<ref>PMID:24598042</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4nj2" style="background-color:#fffaf0;"></div>

==See Also==
*[[Gcn4|Gcn4]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Horne, W S]]
[[Category: Oshaben, K M]]
[[Category: Transcription]]