View source for 4ges


==crystal structure of GFP-TYR151PYZ with an unnatural amino acid incorporation==
<StructureSection load='4ges' size='340' side='right' caption='[[4ges]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ges]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GES FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=0WZ:3-(1H-PYRAZOL-1-YL)-L-TYROSINE'>0WZ</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gf6|4gf6]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ges FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ges OCA], [http://pdbe.org/4ges PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ges RCSB], [http://www.ebi.ac.uk/pdbsum/4ges PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ges ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
ET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu(II) ion was shown to occur within one nanosecond in a distance-dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to Cu(II) ions.

Genetic incorporation of a metal-chelating amino Acid as a probe for protein electron transfer.,Liu X, Li J, Dong J, Hu C, Gong W, Wang J Angew Chem Int Ed Engl. 2012 Oct 8;51(41):10261-5. doi: 10.1002/anie.201204962., Epub 2012 Aug 31. PMID:22936654<ref>PMID:22936654</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ges" style="background-color:#fffaf0;"></div>

==See Also==
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aeqvi]]
[[Category: Dong, J]]
[[Category: Gong, W]]
[[Category: Li, J]]
[[Category: Liu, X]]
[[Category: Wang, J]]
[[Category: Electron transduction]]
[[Category: Fluorescence]]
[[Category: Fluorescent protein]]