View source for 4owv


==Anthranilate phosphoribosyl transferase from Mycobacterium tuberculosis in complex with anthranilate==
<StructureSection load='4owv' size='340' side='right' caption='[[4owv]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4owv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OWV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BE2:2-AMINOBENZOIC+ACID'>BE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4owm|4owm]], [[4own|4own]], [[4owo|4owo]], [[4owq|4owq]], [[4ows|4ows]], [[4owu|4owu]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT2248,MTCY190.03c,Rv2192c,trpD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4owv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4owv OCA], [http://pdbe.org/4owv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4owv RCSB], [http://www.ebi.ac.uk/pdbsum/4owv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4owv ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Anthranilate phosphoribosyltransferase (AnPRT), required for the biosynthesis of tryptophan, is essential for the virulence of Mycobacterium tuberculosis (Mtb). AnPRT catalyses the Mg2+-dependent transfer of a phosphoribosyl group from 5'-phosphoribosyl 1'-pyrophosphate (PRPP) to anthranilate to form 5'-phosphoribosyl anthranilate (PRA). Mtb-AnPRT was shown to catalyse a sequential reaction and significant substrate inhibition by anthranilate was observed. Antimycobacterial fluoroanthranilates and methyl-substituted analogues were shown to act as alternative substrates for Mtb-AnPRT, producing the corresponding substituted PRA products. Structures of the enzyme complexed with anthranilate analogues reveal two distinct binding sites for anthranilate. One site is located over 8 A from PRPP at the entrance to a tunnel leading to the active site, whereas in the second, inner site anthranilate is adjacent to PRPP, in a catalytically relevant position. Soaking of the analogues for variable time periods provides evidence for anthranilate located at transient positions during transfer from the outer site to the inner catalytic site. PRPP and Mg2+ binding have been shown to be associated with the rearrangement of two flexible loops, which is required to complete the inner anthranilate binding site. It is proposed that anthranilate first binds to the outer site, providing an unusual mechanism for substrate capture and efficient transfer to the catalytic site following the binding of PRPP.

Alternative substrates reveal catalytic cycle and key binding events in the reaction catalysed by anthranilate phosphoribosyltransferase from Mycobacterium tuberculosis.,Cookson TV, Castell A, Bulloch EM, Evans GL, Short FL, Baker EN, Lott JS, Parker EJ Biochem J. 2014 Apr 9. PMID:24712732<ref>PMID:24712732</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4owv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Phosphoribosyltransferase|Phosphoribosyltransferase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Anthranilate phosphoribosyltransferase]]
[[Category: Castell, A]]
[[Category: Cookson, T V.M]]
[[Category: Lott, J S]]
[[Category: Short, F L]]
[[Category: Anthranilic acid]]
[[Category: Inhibitor]]
[[Category: Magnesium]]
[[Category: Transferase]]
[[Category: Tryptophan]]