View source for 4c22


==L-Fucose Isomerase In Complex With Fuculose==
<StructureSection load='4c22' size='340' side='right' caption='[[4c22]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4c22]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C22 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CVU:L-FUCULOSE+OPEN+FORM'>CVU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c20|4c20]], [[4c21|4c21]], [[4c23|4c23]], [[4c24|4c24]], [[4c25|4c25]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fucose_isomerase L-fucose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.25 5.3.1.25] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c22 OCA], [http://pdbe.org/4c22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c22 RCSB], [http://www.ebi.ac.uk/pdbsum/4c22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c22 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/FUCI_STRPN FUCI_STRPN]] Converts the aldose L-fucose into the corresponding ketose L-fuculose (By similarity). 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fucose metabolism pathways are present in many bacterial species and typically contain the central fucose-processing enzymes fucose isomerase (FcsI), fuculose kinase (FcsK), and fuculose-1-phosphate aldolase (FcsA). Fucose initially undergoes isomerization by FcsI producing fuculose, which is then phosphorylated by FcsK. FcsA cleaves the fuculose-1-phosphate product into lactaldehyde and dihydroxyacetone phosphate, which can be incorporated into central metabolism allowing the bacterium to use fucose as an energy source. Streptococcus pneumoniae has fucose-processing operons containing homologs of FcsI, FcsK, and FcsA; however, this bacterium appears unable to utilize fucose as an energy source. To investigate this contradiction, we performed biochemical and structural studies of the S. pneumoniae fucose-processing enzymes SpFcsI, SpFcsK, and SpFcsA. These enzymes are demonstrated to act in a sequential manner to ultimately produce dihydroxyacetone phosphate and have structural features entirely consistent with their observed biochemical activities. Analogous to the regulation of the Escherichia coli fucose utilization operon, fuculose-1-phosphate appears to act as an inducing molecule for activation of the S. pneumoniae fucose operon. Despite our evidence that S. pneumoniae appears to have the appropriate regulatory and biochemical machinery for fucose metabolism, we confirmed the inability of the S. pneumoniae TIGR4 strain to grow on fucose or on the H-disaccharide, which is the probable substrate of the transporter for the pathway. On the basis of these observations, we postulate that the S. pneumoniae fucose-processing pathway has a non-metabolic role in the interaction of this bacterium with its human host.

Structural and Functional Analysis of Fucose-Processing Enzymes from Streptococcus pneumoniae.,Higgins MA, Suits MD, Marsters C, Boraston AB J Mol Biol. 2013 Dec 12. pii: S0022-2836(13)00748-1. doi:, 10.1016/j.jmb.2013.12.006. PMID:24333485<ref>PMID:24333485</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4c22" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: L-fucose isomerase]]
[[Category: Strpn]]
[[Category: Boraston, A B]]
[[Category: Higgins, M A]]
[[Category: Marsters, C]]
[[Category: Suits, M D.L]]
[[Category: Fucose processing]]
[[Category: Isomerase]]