View source for 1a42

[[Image:1a42.gif|left|200px]]<br />
<applet load="1a42" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1a42, resolution 2.25&Aring;" />
'''HUMAN CARBONIC ANHYDRASE II COMPLEXED WITH BRINZOLAMIDE'''<br />

==Overview==
Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to, plasma membrane surfaces by a phosphatidylinositol glycan linkage. We have, determined the 2.8-angstroms resolution crystal structure of a truncated, soluble form of recombinant murine CAIV. We have also determined the, structure of its complex with a drug used for glaucoma therapy, the, sulfonamide inhibitor brinzolamide (Azopt). The overall structure of, murine CAIV is generally similar to that of human CAIV; however, some, local structural differences are found in the active site resulting from, amino acid sequence differences in the "130's segment" and the residue-63, loop (these may affect the nearby catalytic proton shuttle, His-64)., Similar to human CAIV, the C-terminus of murine CAIV is surrounded by a, substantial electropositive surface potential that may stabilize the, interaction with the phospholipid membrane. Binding interactions observed, for brinzolamide rationalize the generally weaker affinity of inhibitors, used in glaucoma therapy toward CAIV compared with CAII.

==About this Structure==
1A42 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HG, ZN and BZU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Structure known Active Site: ZN. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A42 OCA]. 

==Reference==
Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: molecular basis of isozyme-drug discrimination., Stams T, Chen Y, Boriack-Sjodin PA, Hurt JD, Liao J, May JA, Dean T, Laipis P, Silverman DN, Christianson DW, Protein Sci. 1998 Mar;7(3):556-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9541386 9541386]
[[Category: Carbonate dehydratase]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Boriack-Sjodin, P.A.]]
[[Category: Christianson, D.W.]]
[[Category: BZU]]
[[Category: HG]]
[[Category: ZN]]
[[Category: inhibitor]]
[[Category: lyase]]
[[Category: zinc]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 15:44:16 2007''